Evaluation of CTB-sLip for Targeting Lung Metastasis of Colorectal Cancer
Xiaoying Zhang,
Wenjing Tang,
Haoyu Wen,
Ercan Wu,
Tianhao Ding,
Jie Gu,
Zhongwei Lv,
Changyou Zhan
Affiliations
Xiaoying Zhang
Department of Nuclear Medicine, The Affiliated Shanghai Tenth People’s Hospital of Nanjing Medical University, Shanghai 200072, China
Wenjing Tang
Center of Medical Research and Innovation, Shanghai Pudong Hospital & Department of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 201399, China
Haoyu Wen
Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Ercan Wu
School of Pharmacy, Fudan University & Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai 201203, China
Tianhao Ding
Center of Medical Research and Innovation, Shanghai Pudong Hospital & Department of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 201399, China
Jie Gu
Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Zhongwei Lv
Department of Nuclear Medicine, The Affiliated Shanghai Tenth People’s Hospital of Nanjing Medical University, Shanghai 200072, China
Changyou Zhan
Center of Medical Research and Innovation, Shanghai Pudong Hospital & Department of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 201399, China
Lung metastasis of colorectal cancer is common in the clinic; however, precise targeting for the diagnosis and therapy purposes of those lung metastases remains challenging. Herein, cholera toxin subunit b (CTB) protein was chemically conjugated on the surface of PEGylated liposomes (CTB-sLip). Both human-derived colorectal cancer cell lines, HCT116 and HT-29, demonstrated high binding affinity and cellular uptake with CTB-sLip. In vivo, CTB-sLip exhibited elevated targeting capability to the lung metastasis of colorectal cancer in the model nude mice in comparison to PEGylated liposomes (sLip) without CTB modification. CTB conjugation induced ignorable effects on the interaction between liposomes and plasma proteins but significantly enhanced the uptake of liposomes by numerous blood cells and splenic cells, leading to relatively rapid blood clearance in BALB/c mice. Even though repeated injections of CTB-sLip induced the production of anti-CTB antibodies, our results suggested CTB-sLip as promising nanocarriers for the diagnosis of lung metastasis of colorectal cancer.
