Isolation and Determination of Bile Salt Hydrolase-Producing Lactic Acid Bacteria from Fermented Spider Plant

Abstract

Bile salt hydrolase is an enzyme from bacteria that can hydrolyze bile acid to be insoluble form and this event lead to reducing of cholesterol level in serum. A new strain of lactic acid bacteria, which was isolated from fermented spider plant in local market of Thailand, showed ability to produce bile salt hydrolase and has potential to be probiotic bacteria with good colonizing properties. The new strain was identified as Lactobacillus plantarum and gene that responsible for bile salt hydrolase production is located on plasmid. From the study could be suggested that novel strain of LAB possible to be further used as cholesterol lowering probiotic bacteria and fermented spider plant would be cheap price cholesterol lowering foods for people in local area.

Keywords

• bile salt
• hydrolase
• lactic acid bacteria