Generation of six human induced pluripotent stem cell sublines (MZT01E, MZT01F, MZT01N and MZT02D, MZT02G and MZT02H) for reproductive science research

Erica C. Pandolfi; Enrique Sosa; Timothy J. Hunt; Sierra Goldsmith; Kellie Hurlbut; Sherman J. Silber; Amander T. Clark

Stem Cell Research (Mar 2021)

Generation of six human induced pluripotent stem cell sublines (MZT01E, MZT01F, MZT01N and MZT02D, MZT02G and MZT02H) for reproductive science research

Erica C. Pandolfi,
Enrique Sosa,
Timothy J. Hunt,
Sierra Goldsmith,
Kellie Hurlbut,
Sherman J. Silber,
Amander T. Clark

Affiliations

Erica C. Pandolfi: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA
Enrique Sosa: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA
Timothy J. Hunt: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA
Sierra Goldsmith: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Kellie Hurlbut: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Sherman J. Silber: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Amander T. Clark: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; Corresponding author.

Journal volume & issue: Vol. 51
p. 102204

Abstract

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Six human induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal fibroblasts (HDFs) derived from skin biopsies donated from monozygotic twin women wherein one woman had proven fertility and her sister was infertile due to ovarian failure. Three hiPSC sublines were created from each twin’s HDFs. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using PluriTest. We show here that the hiPSC lines created from the twins are equivalent in measures of pluripotency and self-renewal, despite their differential diagnosis.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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