Nature Communications (May 2016)

MNase titration reveals differences between nucleosome occupancy and chromatin accessibility

  • Jakub Mieczkowski,
  • April Cook,
  • Sarah K. Bowman,
  • Britta Mueller,
  • Burak H. Alver,
  • Sharmistha Kundu,
  • Aimee M. Deaton,
  • Jennifer A. Urban,
  • Erica Larschan,
  • Peter J. Park,
  • Robert E. Kingston,
  • Michael Y. Tolstorukov

DOI
https://doi.org/10.1038/ncomms11485
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 11

Abstract

Read online

Nucleosome positioning and chromatin accessibility are important contributors to the regulation of gene expression. Here the authors describe a method that allows the simultaneous measurement of nucleosome occupancy and chromatin accessibility in the same assay, revealing new features of chromatin organization linked to gene regulation.