MNase titration reveals differences between nucleosome occupancy and chromatin accessibility

Jakub Mieczkowski; April Cook; Sarah K. Bowman; Britta Mueller; Burak H. Alver; Sharmistha Kundu; Aimee M. Deaton; Jennifer A. Urban; Erica Larschan; Peter J. Park; Robert E. Kingston; Michael Y. Tolstorukov

doi:10.1038/ncomms11485

Nature Communications (May 2016)

MNase titration reveals differences between nucleosome occupancy and chromatin accessibility

Jakub Mieczkowski,
April Cook,
Sarah K. Bowman,
Britta Mueller,
Burak H. Alver,
Sharmistha Kundu,
Aimee M. Deaton,
Jennifer A. Urban,
Erica Larschan,
Peter J. Park,
Robert E. Kingston,
Michael Y. Tolstorukov

Affiliations

Jakub Mieczkowski: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School
April Cook: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School
Sarah K. Bowman: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School
Britta Mueller: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School
Burak H. Alver: Department of Biomedical Informatics, Harvard Medical School
Sharmistha Kundu: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School
Aimee M. Deaton: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School
Jennifer A. Urban: Department of Molecular Biology, Cell Biology and Biochemistry, Brown University
Erica Larschan: Department of Molecular Biology, Cell Biology and Biochemistry, Brown University
Peter J. Park: Department of Biomedical Informatics, Harvard Medical School
Robert E. Kingston: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School
Michael Y. Tolstorukov: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School

DOI: https://doi.org/10.1038/ncomms11485
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 11

Abstract

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Nucleosome positioning and chromatin accessibility are important contributors to the regulation of gene expression. Here the authors describe a method that allows the simultaneous measurement of nucleosome occupancy and chromatin accessibility in the same assay, revealing new features of chromatin organization linked to gene regulation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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