Проблемы особо опасных инфекций (Mar 2017)

BIOLOGICAL PROPERTIES AND MOLECULAR-GENETIC CHARACTERISTICS OF BACILLUS ANTHRACIS STRAINS, ISOLATED DURING THE OUTBREAK OF ANTHRAX IN THE YAMALO-NENETS AUTONOMOUS DISTRICT IN 2016

  • A. N. Kulichenko,
  • E. I. Eremenko,
  • A. G. Ryazanova,
  • L. Yu. Aksenova,
  • D. A. Kovalev,
  • S. V. Pisarenko,
  • N. G. Varfolomeeva,
  • A. M. Zhirov,
  • А. S. Volynkina,
  • N. P. Buravtseva,
  • T. M. Golovinskaya,
  • E. A. Koteneva,
  • O. I. Tsygankova,
  • I. A. Dyatlov,
  • V. S. Timofeev,
  • A. G. Bogun,
  • I. V. Bakhteeva,
  • A. A. Kislichkina,
  • R. I. Mironova,
  • G. M. Titareva,
  • Yu. P. Skryabin,
  • Yu. O. Selyaninov,
  • I. Yu. Egorova,
  • D. V. Kolbasov

DOI
https://doi.org/10.21055/0370-1069-2017-1-94-99
Journal volume & issue
Vol. 0, no. 1
pp. 94 – 99

Abstract

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Objective of the study was to identify phenotypic properties and genetic peculiarities of Bacillus anthracis strains, isolated during the outbreak of anthrax in the territory of Yamal in 2016. Materials and methods. Investigated were the strains of anthrax agent, applying basic and subsequent identification tests and canSNP-, MLVA-genotyping methods and whole genome sequencing. Results and conclusions. The results showed the identity of the phenotypic properties, canSNPand MLVA25-genotypes, and profiles of whole genome-sequencing, regardless of the source of the strains isolation. Confirmed was a common source of human infection. Defined were phylogenetic interrelations of the tested strains and their position in global B. anthracis population. For the first time ever explored was variability of the gene pattern, associated with pathogenicity, and demonstrated – the efficiency of the proposed algorithm for genetic typing.

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