Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats

Jan-Erik Werry; Stefan Müller; Falk Wehrhan; Carol Geppert; Gesche Frohwitter; Jutta Ries; Peer W. Kämmerer; Tobias Moest; Rainer Lutz; Andi Homm; Marco Kesting; Manuel Weber

doi:10.3390/app11125589

Applied Sciences (Jun 2021)

Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats

Jan-Erik Werry,
Stefan Müller,
Falk Wehrhan,
Carol Geppert,
Gesche Frohwitter,
Jutta Ries,
Peer W. Kämmerer,
Tobias Moest,
Rainer Lutz,
Andi Homm,
Marco Kesting,
Manuel Weber

Affiliations

Jan-Erik Werry: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Stefan Müller: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Falk Wehrhan: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Carol Geppert: Department of Pathology, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Gesche Frohwitter: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Jutta Ries: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Peer W. Kämmerer: Department of Oral and Maxillofacial Surgery, University of Mainz, 55131 Mainz, Germany
Tobias Moest: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Rainer Lutz: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Andi Homm: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Marco Kesting: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
Manuel Weber: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany

DOI: https://doi.org/10.3390/app11125589
Journal volume & issue: Vol. 11, no. 12
p. 5589

Abstract

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This study aimed to investigate accuracy in different sectional planes of the TMA Grand Master (3DHISTECH) Workstation in various soft tissue samples collected from Wistar rats. A total of 108 animals were sacrificed and 963 tissue specimens collected from 12 soft-tissue types. A total of 3307 tissue cores were punched and transferred into 40 recipient TMA blocks. Digital image analysis was performed. Core loss showed a significant correlation with tissue type and was highest in skin tissue (p p ®-method has proven accurate and reliable in different soft tissues, even in deeper sectional layers.

Published in Applied Sciences

ISSN: 2076-3417 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Engineering (General). Civil engineering (General); Science: Biology (General); Science: Physics; Science: Chemistry
Website: http://www.mdpi.com/journal/applsci

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