Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.)

Abstract

Until now in Europe has not been cultivated any hybrid cultivar of oilseed rape based on the Shaan 2A cytoplasmic male sterility (CMS), a widely used CMS system in China. The aim of Czech breeders now is to produce new, improved cultivars of rapeseed based on this CMS system. Sterile Shaan 2A CMS line (S; rf/rf), its corresponding maintainers (N; rf/rf) and fertility restorers (S; Rf/Rf) were analyzed on molecular level for the presence of functional CMS cytoplasm. Two new primer pairs covering CMS-associated gene (so called orf224-1) present in Shaan 2A CMS line were developed and selection capability of the developed primers was successfully evaluated. These primers can be used for early selection of plants with functional Shaan 2A CMS system in breeding programmes.

Keywords

• cytoplasmic male sterility (cms)
• marker-assisted selection (mas)
• orf224-1
• rapeseed (brassica napus l.)
• rf-restorer gene
• shaan 2a cms line