Characterization and Functional Studies of a Novel Depolymerase Against K19-Type Klebsiella pneumoniae

Yunfen Hua; Yunfen Hua; Yongqin Wu; Yongqin Wu; Minjie Guo; Ruijing Ma; Ruijing Ma; Qingchuan Li; Zheyuan Hu; Hongrui Chen; Xingyu Zhang; Hui Li; Qingtian Li; Ping He; Ping He

doi:10.3389/fmicb.2022.878800

Frontiers in Microbiology (Jun 2022)

Characterization and Functional Studies of a Novel Depolymerase Against K19-Type Klebsiella pneumoniae

Yunfen Hua,
Yunfen Hua,
Yongqin Wu,
Yongqin Wu,
Minjie Guo,
Ruijing Ma,
Ruijing Ma,
Qingchuan Li,
Zheyuan Hu,
Hongrui Chen,
Xingyu Zhang,
Hui Li,
Qingtian Li,
Ping He,
Ping He

Affiliations

Yunfen Hua: College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China
Yunfen Hua: Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Yongqin Wu: College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China
Yongqin Wu: Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Minjie Guo: Department of Nanoengineering, University of California, San Diego, La Jolla, CA, United States
Ruijing Ma: Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Ruijing Ma: NHC Key Laboratory of Parasite and Vector Biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
Qingchuan Li: Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Zheyuan Hu: Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Hongrui Chen: Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Xingyu Zhang: Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Hui Li: Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Qingtian Li: Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Ping He: Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Ping He: NHC Key Laboratory of Parasite and Vector Biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China

DOI: https://doi.org/10.3389/fmicb.2022.878800
Journal volume & issue: Vol. 13

Abstract

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Carbapenem-resistant Klebsiella pneumoniae (CRKP), a pathogen that causes severe nosocomial infections and yields a high mortality rate, poses a serious threat to global public health due to its high antimicrobial resistance. Bacteriophages encode polysaccharide-degrading enzymes referred to as depolymerases that cleave the capsular polysaccharide (CPS), one of the main virulence factors of K. pneumoniae. In this study, we identified and characterized a new capsule depolymerase K19-Dpo41 from K. pneumoniae bacteriophage SH-KP156570. Our characterization of K19-Dpo41 demonstrated that this depolymerase showed specific activities against K19-type K. pneumoniae. K19-Dpo41-mediated treatments promoted the sensitivity of a multidrug-resistant K19-type K. pneumoniae strain to the bactericidal effect of human serum and significantly increased the survival rate of Galleria mellonella infected with K19-type K. pneumoniae. Our results provided strong primary evidence that K19-Dpo41 was not only effective in capsular typing of K19-type K. pneumoniae but promising in terms of developing new alternative therapeutic strategies against K19-type CRKP infections in the future.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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