Nature Communications (Oct 2021)

Improvement of a synthetic live bacterial therapeutic for phenylketonuria with biosensor-enabled enzyme engineering

  • Kristin J. Adolfsen,
  • Isolde Callihan,
  • Catherine E. Monahan,
  • Per Jr. Greisen,
  • James Spoonamore,
  • Munira Momin,
  • Lauren E. Fitch,
  • Mary Joan Castillo,
  • Lindong Weng,
  • Lauren Renaud,
  • Carl J. Weile,
  • Jay H. Konieczka,
  • Teodelinda Mirabella,
  • Andres Abin-Fuentes,
  • Adam G. Lawrence,
  • Vincent M. Isabella

DOI
https://doi.org/10.1038/s41467-021-26524-0
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 13

Abstract

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PKU patients have elevated phenylalanine levels which can result in neurological impairment. Here the authors utilize biosensor-based ultra-high-throughput screening to optimize PAL activity in a synthetic biotic platform for improved in vivo performance.