Pharmacological Research - Modern Chinese Medicine (Sep 2023)
Effects of hesperetin on dimethylsulphoxide induced cognitive dysfunction mediated through oxidative stress marker and neuroinflammation in hippocampus
Abstract
Objective: Hesperetin is a natural flavonoid with antioxidant potential and can be used to treat disorders of the brain. Therefore, present study is designed to investigate effect of hesperetin on dimethylsulphoxide induced cognitive dysfunction mediated through oxidative stress marker and neuroinflammation in hippocampus Methodology: The experiment was conducted using five treatment groups, each group consisting six rats. Group 1 (control) received 1 ml/kg normal saline interperitoneally for 14 days. Group 2 received 1 ml/kg DMSO per oral for14 days. Group 3 received donepezil 3 mg/kg per day for 14 days and DMSO (1 ml/kg p.o.) for 14th day. Group 4 and 5 received a dose of 25 mg /kg of hesperetin (H1) and 50 mg/kg hesperetin (H2) per oral, per day for 14 days, respectively. Hesperetin was dissolved in DMSO to get 25 mg/ml solution. On 14th day, the memory of rats was assessed by Cook's pole climbing apparatus and Morris water maze test. On 15th day, six rats of each group were sacrificed and the isolated brain tissues were subjected for the estimation of biochemical parameters, beta actin blot analysis and histopathological examination. Results: The DMSO treated rats showed significantly enhanced escape latency time (P < 0.0001) acetylcholinesterase (AchE) activity (P < 0.0001), lipid peroxidation (P < 0.0001), TNF-α level and decreased reduced glutathione levels (p < 0.001), attenuated beta actin blot density, degradation and inflammation in the hippocampus region as compared to the control group. These parameters were significantly recovered in the rats pretreated with donepezil and hesperetin. Conclusion: The findings of this study demonstrated that DMSO induced cognitive dysfunction in rats and hesperetin has potential to improve the cognitive performance against DMSO via reduced oxidative stress and neuroinflammation in hippocampus.
