Effect of Fuzheng Jiedu granule on immunological function and level of immune-related cytokines in immune-suppressed mice

Abstract

Fuzheng Jiedu granule exhibits a number of health benefits and it is thought that the mechanisms involved in these effects are due to the modulation of immunity. In this article, we studied the effect of Fuzheng Jiedu granule on immunological function and the expression of immune-related cytokines in immune-suppressed mice. 72 mice were randomly divided into six groups, with 12 in each group. The control groups included an untreated group, a negative control group (Cyclophosphamide) and a positive control group (Astragalus polysaccharide). There were three treated groups, which were given different doses of Fuzheng Jiedu granule: a low dose (100 mg kg–1), a medium dose (400 mg kg–1) and a high dose (600 mg kg–1). With the exception of the untreated control animals, each group received an intraperitoneal injection of Cyclophosphamide (100 mg kg–1) for 3 days to establish the immune-suppressed model. Mice were then treated for 19 consecutive days and, 24 h after the last treatment, blood was taken for the eyeballs and serum separation was performed. Analysis was made of the levels of related cytokines (IgA, IgG, IgM, IL-6, IFN-γ, C3, C4 and TNF-α), the transformation of lymphocytes and the immune organ indexes. The results showed that Fuzheng Jiedu granule can improve the levels of cytokines, the rate of proliferation of lymphocytes and the immune organ indexes of immune-suppressed mice.

Keywords

• Fuzheng Jiedu granule
• immune suppression
• cytokines
• lymphocytes
• immune organ index