When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing

Vladimir Lazarevic; Nadia Gaïa; Myriam Girard; Stefano Leo; Abdessalam Cherkaoui; Gesuele Renzi; Stéphane Emonet; Sharon Jamme; Etienne Ruppé; Sandrine Vijgen; Laura Rubbia-Brandt; Christian Toso; Jacques Schrenzel; Jacques Schrenzel

doi:10.3389/fmicb.2018.01566

Frontiers in Microbiology (Jul 2018)

When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing

Vladimir Lazarevic,
Nadia Gaïa,
Myriam Girard,
Stefano Leo,
Abdessalam Cherkaoui,
Gesuele Renzi,
Stéphane Emonet,
Sharon Jamme,
Etienne Ruppé,
Sandrine Vijgen,
Laura Rubbia-Brandt,
Christian Toso,
Jacques Schrenzel,
Jacques Schrenzel

Affiliations

Vladimir Lazarevic: Genomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Nadia Gaïa: Genomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Myriam Girard: Genomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Stefano Leo: Genomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Abdessalam Cherkaoui: Bacteriology Laboratory, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Gesuele Renzi: Bacteriology Laboratory, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Stéphane Emonet: Bacteriology Laboratory, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Sharon Jamme: Department of Community, Primary Care and Emergency Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Etienne Ruppé: Genomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Sandrine Vijgen: Division of Clinical Pathology, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Laura Rubbia-Brandt: Division of Clinical Pathology, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Christian Toso: Department of Surgery, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Jacques Schrenzel: Genomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland
Jacques Schrenzel: Bacteriology Laboratory, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland

DOI: https://doi.org/10.3389/fmicb.2018.01566
Journal volume & issue: Vol. 9

Abstract

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Here, we sequenced DNA extracted from a necrotic hepatic lesion from a patient with suspected chronic hepatic brucelloma but negative culture results. Although most of the taxonomically classified sequencing reads corresponded to human genome sequences, our data suggest that whole-metagenome shotgun sequencing may be used together with other tests to strengthen the diagnosis of hepatic brucelloma.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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Abstract

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