Nature Communications (Jul 2020)
Raman image-activated cell sorting
- Nao Nitta,
- Takanori Iino,
- Akihiro Isozaki,
- Mai Yamagishi,
- Yasutaka Kitahama,
- Shinya Sakuma,
- Yuta Suzuki,
- Hiroshi Tezuka,
- Minoru Oikawa,
- Fumihito Arai,
- Takuya Asai,
- Dinghuan Deng,
- Hideya Fukuzawa,
- Misa Hase,
- Tomohisa Hasunuma,
- Takeshi Hayakawa,
- Kei Hiraki,
- Kotaro Hiramatsu,
- Yu Hoshino,
- Mary Inaba,
- Yuki Inoue,
- Takuro Ito,
- Masataka Kajikawa,
- Hiroshi Karakawa,
- Yusuke Kasai,
- Yuichi Kato,
- Hirofumi Kobayashi,
- Cheng Lei,
- Satoshi Matsusaka,
- Hideharu Mikami,
- Atsuhiro Nakagawa,
- Keiji Numata,
- Tadataka Ota,
- Takeichiro Sekiya,
- Kiyotaka Shiba,
- Yoshitaka Shirasaki,
- Nobutake Suzuki,
- Shunji Tanaka,
- Shunnosuke Ueno,
- Hiroshi Watarai,
- Takashi Yamano,
- Masayuki Yazawa,
- Yusuke Yonamine,
- Dino Di Carlo,
- Yoichiroh Hosokawa,
- Sotaro Uemura,
- Takeaki Sugimura,
- Yasuyuki Ozeki,
- Keisuke Goda
Affiliations
- Nao Nitta
- Department of Chemistry, The University of Tokyo
- Takanori Iino
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Akihiro Isozaki
- Department of Chemistry, The University of Tokyo
- Mai Yamagishi
- Department of Biological Sciences, The University of Tokyo
- Yasutaka Kitahama
- Department of Chemistry, The University of Tokyo
- Shinya Sakuma
- Department of Micro-Nano Mechanical Science and Engineering, Nagoya University
- Yuta Suzuki
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Hiroshi Tezuka
- Department of Creative Informatics, The University of Tokyo
- Minoru Oikawa
- Natural Sciences Cluster, Sciences Unit, Kochi University
- Fumihito Arai
- Department of Micro-Nano Mechanical Science and Engineering, Nagoya University
- Takuya Asai
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Dinghuan Deng
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Hideya Fukuzawa
- Graduate School of Biostudies, Kyoto University
- Misa Hase
- Department of Chemistry, The University of Tokyo
- Tomohisa Hasunuma
- Graduate School of Science, Technology and Innovation, Kobe University
- Takeshi Hayakawa
- Department of Precision Mechanics, Chuo University
- Kei Hiraki
- Department of Chemistry, The University of Tokyo
- Kotaro Hiramatsu
- Department of Chemistry, The University of Tokyo
- Yu Hoshino
- Department of Chemical Engineering, Kyushu University
- Mary Inaba
- Department of Creative Informatics, The University of Tokyo
- Yuki Inoue
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Takuro Ito
- Department of Chemistry, The University of Tokyo
- Masataka Kajikawa
- Graduate School of Biostudies, Kyoto University
- Hiroshi Karakawa
- Department of Chemistry, The University of Tokyo
- Yusuke Kasai
- Department of Micro-Nano Mechanical Science and Engineering, Nagoya University
- Yuichi Kato
- Engineering Biology Research Center, Kobe University
- Hirofumi Kobayashi
- Department of Chemistry, The University of Tokyo
- Cheng Lei
- Department of Chemistry, The University of Tokyo
- Satoshi Matsusaka
- Department of Clinical Research and Regional Innovation, University of Tsukuba
- Hideharu Mikami
- Department of Chemistry, The University of Tokyo
- Atsuhiro Nakagawa
- Department of Neurosurgery, Graduate School of Medicine, Tohoku University
- Keiji Numata
- Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science
- Tadataka Ota
- Department of Chemistry, The University of Tokyo
- Takeichiro Sekiya
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Kiyotaka Shiba
- Division of Protein Engineering, Cancer Institute of Japanese Foundation for Cancer Research
- Yoshitaka Shirasaki
- Department of Biological Sciences, The University of Tokyo
- Nobutake Suzuki
- Department of Biological Sciences, The University of Tokyo
- Shunji Tanaka
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Shunnosuke Ueno
- Department of Chemistry, The University of Tokyo
- Hiroshi Watarai
- Institute of Medical Science, The University of Tokyo
- Takashi Yamano
- Graduate School of Biostudies, Kyoto University
- Masayuki Yazawa
- Department of Rehabilitation and Regenerative Medicine and Pharmacology, Columbia University
- Yusuke Yonamine
- Research Institute for Electronic Science, Hokkaido University
- Dino Di Carlo
- Department of Chemistry, The University of Tokyo
- Yoichiroh Hosokawa
- Division of Materials Science, Nara Institute of Science and Technology
- Sotaro Uemura
- Department of Biological Sciences, The University of Tokyo
- Takeaki Sugimura
- Department of Chemistry, The University of Tokyo
- Yasuyuki Ozeki
- Department of Electrical Engineering and Information Systems, The University of Tokyo
- Keisuke Goda
- Department of Chemistry, The University of Tokyo
- DOI
- https://doi.org/10.1038/s41467-020-17285-3
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 16
Abstract
Most current cell sorting methods are based on fluorescence detection with no imaging capability. Here the authors generate and use Raman image-activated cell sorting with a throughput of around 100 events per second, providing molecular images with no need for labeling.
