Molecules (Jul 2023)

Cellular Antioxidant, Anti-Inflammatory, and Antiproliferative Activities from the Flowers, Leaves and Fruits of <i>Gallesia integrifolia</i> Spreng Harms

  • Gabriela Catuzo Canônico Silva,
  • Mariane de Almeida Machado,
  • Karina Sakumoto,
  • Rodrigo Sadao Inumaro,
  • José Eduardo Gonçalves,
  • Filipa Mandim,
  • Josiana Vaz,
  • Juliana Silveira do Valle,
  • Maria Graciela Iecher Faria,
  • Suelen Pereira Ruiz,
  • Ranulfo Piau Junior,
  • Daniela Dib Gonçalves,
  • Zilda Cristiani Gazim

DOI
https://doi.org/10.3390/molecules28145406
Journal volume & issue
Vol. 28, no. 14
p. 5406

Abstract

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Gallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC–MS and CEs by UHPLC–MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2′-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI50 between 51 and 230 µg/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 µg/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.

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