Exploring Borate-Modified Calcium Phosphate Ceramics: Antimicrobial Potential and Cytocompatibility Assessment
Inna V. Fadeeva,
Katia Barbaro,
Annalisa Altigeri,
Anna A. Forysenkova,
Marat R. Gafurov,
Georgy V. Mamin,
Alexander V. Knot’ko,
Viktoriya G. Yankova,
Anna A. Zhukova,
Fabrizio Russo,
Julietta V. Rau
Affiliations
Inna V. Fadeeva
A.A. Baikov Institute of Metallurgy and Material Science, Russian Academy of Sciences, Leninsky Prospect 49, 119334 Moscow, Russia
Katia Barbaro
Istituto Zooprofilattico Sperimentale Lazio e Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, Italy
Annalisa Altigeri
Istituto Zooprofilattico Sperimentale Lazio e Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, Italy
Anna A. Forysenkova
A.A. Baikov Institute of Metallurgy and Material Science, Russian Academy of Sciences, Leninsky Prospect 49, 119334 Moscow, Russia
Marat R. Gafurov
Institute of Physics, Kazan Federal University, Kremlyovskaya St. 18, 420008 Kazan, Russia
Georgy V. Mamin
Institute of Physics, Kazan Federal University, Kremlyovskaya St. 18, 420008 Kazan, Russia
Alexander V. Knot’ko
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia
Viktoriya G. Yankova
Department of Analytical, Physical and Colloid Chemistry, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Build. 2, 119048 Moscow, Russia
Anna A. Zhukova
Department of Analytical, Physical and Colloid Chemistry, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Build. 2, 119048 Moscow, Russia
Fabrizio Russo
Laboratory of Regenerative Orthopaedics, Research Unit of Orthopaedic, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Via Alvaro del Portillo 21, 00128 Rome, Italy
Julietta V. Rau
Department of Analytical, Physical and Colloid Chemistry, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Build. 2, 119048 Moscow, Russia
Addressing periprosthetic infections, which present significant healing challenges that often require revision surgeries, necessitates the development of novel antibacterial materials and implants. Current research focuses on creating materials that hinder bacterial adhesion, colonization, and proliferation in surrounding tissues. Boron (B)-containing compounds are known for their antibacterial properties and potential in bone metabolism for regenerative medicine. In this study, we synthesized B-containing tricalcium phosphate (0.3B-TCP) with 1.1 wt.% B content via precipitation from aqueous solutions and sintering at 1100 °C. X-ray diffraction confirmed the ceramic’s primary crystalline phase as β-TCP, with B evenly distributed according to energy-dispersive spectroscopy data. Electron paramagnetic resonance (EPR) data verified stable paramagnetic borate anions, indicating successful BO33− substitution for phosphate groups. The microstructural properties of 0.3B-TCP ceramic were assessed before and after soaking in a saline solution. Its bending strength was approximately 30 MPa, and its porosity was about 33%. 0.3B-TCP ceramic demonstrated significant antimicrobial efficacy against various bacterial strains and a fungus. Cytotoxicity evaluation using equine adipose tissue-derived mesenchymal stem cells and osteogenic differentiation assessment were conducted. The combination of antibacterial efficacy and good cytocompatibility suggests 0.3B-TCP ceramic as a promising bone substitute material.
