Influence of arbuscular mycorrhiza fungi, rice-husk-drived biochar and compost on dry matter yield, nutrients uptake and secondary metabolites responses of Iranian borage <em>Echium amoenum</em> Fisch & C. A. Mey

Abstract

This study was carried out to investigate the effect of bio-fertilizers including mycorrhiza (MY), rice husk compost (RHC), and biochar (RHB) on dry matter yield, nutrients uptake and some secondary metabolites of the medicinal plant Echium amoenum Fisch & C. A. Mey. The experiment was conducted in a completely randomized design and executed with six treatments and six replications. Treatments comprised of T1: control, T2: MY, T3: RHC, T4: RHB, T5: RHC+MY and T6: RHB+MY. The following parameters were studied: leaf dry weight, macro and micro nutrient uptake, chlorophyll a, chlorophyll b, carotenoids, proline, anthocyanin, flavonoid, mucilage and carbohydrate content. The results show that application of RHC, RHB and MY individually or in combination significantly affected the studied parameters in comparison with the control treatment. In all cases, combined appli­cation of bio-fertilizers together with mycorrhiza application (T5 and T6) had a more positive impact on the studied parameters compared to the application of each treatment alone.

Keywords

• Bio-fertilizers
• Nutrient uptake
• Dry matter yield
• Secondary metabolites