Effect of posphate solubilizing bacteria inoculation on Maple (Acer velutinum) seedlings

Abstract

Soil microorganisms are important components of forest ecosystems. The term plant growth promoting rhizobacteria (PGPR) is used to describe non-symbiotic soil bacteria, which have potential to colonize plant root and stimulate growth. The aim of study was assessment the effects of Acer velutinum seeds inoculation with PSBs. Five isolated bacterial strains (including Pasturella, Acintobacillus, Pseudomonas, Alcaligense and Enerobacter) were cultured separately in liquid Seperb medium at 25 ºC. Bacterial cells were isolated by centrifuge and resuspended in phosphate buffer saline (pH= 7.2) to a density of 3×108 cfu per ml. Seeds of Acer velutinum were sowed in plastic pots and inoculated with bacterial suspension. Control seeds received sterile phosphate buffer saline. After seven months, seedlings were harvested. Shoot height, root length and their dry matter, available phosphorous in soil and seedlings were determined by standard methods. Results indicated the positive effect of PSBs on seedling emergence of Acer velutinum. Inoculated seedlings had more developed root system than those of controls. In conclusion, results indicated the stimulatory effect of PSBs on seedling emergence and their growth. According to results, it seemed inoculation of a mixed inoculums will be more effective in stimulating of growth. Acer velutinum, PSBs, inoculation, seedling.

Keywords

• Acer velutinum
• PSBs
• Inoculation
• Seedling