Transcriptomic analyses provide new insights into immune response of the Procambarus clarkii intestines to Aeromonas hydrophila challenge

Xin Liu; Dan-Dan Bian; Jun-Jie Jiang; Sheng Tang; Xiang Lu; Dai-Zhen Zhang; Qiu-Ning Liu; Bo-Ping Tang; Li-Shang Dai

Aquaculture Reports (Jun 2024)

Transcriptomic analyses provide new insights into immune response of the Procambarus clarkii intestines to Aeromonas hydrophila challenge

Xin Liu,
Dan-Dan Bian,
Jun-Jie Jiang,
Sheng Tang,
Xiang Lu,
Dai-Zhen Zhang,
Qiu-Ning Liu,
Bo-Ping Tang,
Li-Shang Dai

Affiliations

Xin Liu: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai, China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China; Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, China
Dan-Dan Bian: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai, China
Jun-Jie Jiang: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai, China; Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, China
Sheng Tang: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai, China; Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, China
Xiang Lu: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai, China; Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, China
Dai-Zhen Zhang: Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, China
Qiu-Ning Liu: Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, China; Corresponding authors.
Bo-Ping Tang: Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, China; Corresponding authors.
Li-Shang Dai: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China; Corresponding authors.

Journal volume & issue: Vol. 36
p. 102103

Abstract

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In recent years, the death rate of Procambarus clarkii increased sharply due to pathogenic bacterial infections, leading to huge economic losses. To determine the immune response of the intestinal tissues of P. clarkii to bacterial infections, we compared the immune indexes, histopathological damage, and transcriptome data of the control and Aeromonas hydrophila-infected P. clarkii. With the injection of A. hydrophila, it was found that intestinal mucosal folds increased, intestinal villi showed obvious vacuolation, and the tissue structure changed significantly. The results of non-specific immunoenzyme activity showed that the Total protein (TP) content was significantly lower than that of the control group at 24 h, and the Acid phosphatase (ACP) and Alkaline phosphatase (AKP) activities in the treatment group showed a significant increasing trend (p < 0.05). In terms of the transcriptome, an average of 22,927,523 clean reads and 27,866 genes were found in the control and treatment groups through gene assembly and annotation. Among these genes, 567 were identified as differentially expressed genes (DEGs), of which 501 were up-regulated and 66 were down-regulated. Quantitative-polymerase chain reaction (qPCR) analysis of six randomly selected genes was used to verify the RNA-sequencing results. The Gene Ontology enrichment analysis revealed that the DEGs were primarily involved in cell processes, metabolic processes, and biological regulation, while the Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that these DEGs were involved in tyrosine metabolism, glycosphingolipid biosynthesis—ganglionic series, and nitrogen metabolism. We studied the tyrosine metabolic pathway and found that it contributes to the immune defense mechanism of P. clarkii. The results of our study provide important insights into the intestinal immune response of P. clarkii to A. hydrophila infection.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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