Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved

Abstract

The rapid overcompensatory growth that appears when cyanobacteria are supplied with adequate resources after a period of resource deprivation might contribute to the occurrence of cyanobacterial blooms. We investigated the changing characteristics of overcompensatory growth and serine/threonine kinase (STK) genes expression of cyanobacterium Microcystis aeruginosa in response to light limitation. The results showed M. aeruginosa exhibited overcompensatory growth for 2 days after light recovery, during which the increase in growth was inversely related to light intensity. Expression of STK genes, such as spkD, was upregulated significantly at 0.5–4 h after light recovery (P < 0.05). To investigate the function of STK genes in the overcompensatory growth, M. aeruginosa spkD was heterologously expressed in Synechocystis. Transgenic Synechocystis exhibited greater and longer overcompensatory growth than wild-type Synechocystis after light recovery. Relative expression levels of STK genes in transgenic Synechocystis were significantly higher than those in wild-type Synechocystis at 24 h of light recovery (P < 0.05). Heterologous expression of Microcystis spkD might stimulate overcompensatory growth of Synechocystis by affecting its STK gene expression. HIGHLIGHTS Light limitation induced overcompensatory growth of Microcystis.; Light limitation affected expression of most STK genes of Microcystis.; Heterologous expression of Microcystis spkD enhanced and prolonged light-limitation-induced overcompensatory growth of transgenic Synechocystis.; Heterologous expression of Microcystis spkD stimulated relative expression levels of STK genes in transgenic Synechocystis.;

Keywords

• compensatory growth
• cyanobacteria
• light limitation
• serine/threonine kinase
• spkd