Comparison of head space solid phase microextraction (HS-SPME) and hydro-distillation (HD) techniques in determination of essential oils in Fritillaria imperialis

Abstract

In this research, the essential oil from aerial parts of Fritillaria imperialis was extracted by Head space-Solid phase microextraction (HS-SPME) and analyzed by Gas Chromatography-Mass Spctroscopy (GC-MS) methods. 24 components (86.61%) were identified in the oil was isolated by hydrodistillation. The major components of oil of F.imperialis were α-Bisabolol oxide A (28.2%), Camphor (12.86%), Chamazulene (11.8%), Trans-Thujone (10.9%), α-Bisabolone oxide A (10.3%), δ-3-Carene (6.9%) and α-Pinene (5.65%). 21 components (94.93%) were characterized in the oil which was obtained by Head space-solid phase microextraction (HS-SPME). The major components of the oil were α-Bisabolol oxide A (35.2%), Camphor (9.54%), Chamazulene (18.3%), Trans-Thujone (7.89%), α-Bisabolone oxide A (11.8%), δ-3-Carene (6.5%) and α-Pinene (5.7%). In both methods (HD & HS-SPME), oxygenated sesquiterpenes were the main components of the oil, whereas hydrocarbon sesquiterpens were trace.

Keywords

• <i>fritillaria imperialis</i>
• head space solid phase microextraction (hs-spme)
• hydrodistillation (hd)
• gc-ms.