Physiological Responses to Cadmium, Nickel and their Interaction in the Seedlings of Two Maize (Zea mays L.) Cultivars

Abstract

In the leaves of maize seedlings, cultivars Premia and Blitz, the relatively low 2 μmol/L concentration of cadmium (Cd), nickel (Ni), or both metals acting simultaneously (Cd +Ni) for 72 h, induced a significant metal accumulation, decrease in total K+ content, reduction of light-induced membrane electrical potential (EM) repolarisation in mesophyll cells and changes of ascorbic acid (AsA), dehydroascorbic acid (DHA) and glutathione (GSH) content. Shoot growth and the values of resting EM did not change significantly. Increased K+ leakage, from the leaves, and lipid peroxidation accompanied by increase of TBA-reactive substances (TBARS) were found only in cv. Blitz exposed to Cd + Ni. This indicates a capability of high leaf-cell anti-oxidant pool to ameliorate the toxic effects on plasma membrane of single ions in both cultivars, and of Cd + Ni only in cv. Premia. The decreased total content of K+ in leaves in all variants indicated repressing the K+ uptake and/or distribution to the shoots. Under anoxia, the magnitude of the repolarisation obtained after switching on the light was smaller in Cd-treated cultivar Premia than in the controls, and this also occurred in Ni- and Cd + Ni-treated cultivar Blitz.

Keywords

• maize leaves
• heavy metals (cd, ni)
• plasma membrane integrity
• photoelectric response
• antioxidants