Additions of precursors and elicitors improve geranylgeraniol production in Croton stellatopilosus callus cultures

Abstract

Strategies for enhancing GGOH production in Croton stellatopilosus callus culture included additions of precursors (sodium acetate-NA, sodium pyruvate-NP, mevalonic acid lactone-MVA) and elicitors (methyl jasmonate-MJ, acetylsalicylic acid-ASA, yeast extract-YE). Treated cells were evaluated for their GGOH contents by GC-FID and compared with the nontreated cells as controls. Additions of NA (25 mg/L), NP (50 mg/L) and MVA (100 mg/L) resulted in an enhancement of GGOH productivity to 0.61 mg/g DW, 0.52 mg/g DW and 0.70 mg/g DW, respectively, compared to the control culture (0.29 mg/g DW). Callus cultures elicited with MJ at 30 mg/L for 24 h stimulated GGOH production to 0.35 mg/g DW compared to the control culture (0.07 mg/g DW). Cells also responded to ASA (20 mg/L, 2 days) and YE (0.25 g/L, 4 days) and produced GGOH contents of 0.46 mg/g DW and 1.37 mg/g DW, respectively. This study has shown that isoprenoid precursors and conventional elicitors enhanced GGOH production in the C. stellatopilosus callus culture.

Keywords

• Croton stellatopilosus
• callus culture
• elicitation
• geranylgeraniol
• precursor addition