Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Yanxiong Pan; Hui Li; Qiaobin Li; Mary Lenertz; Isabelle Schuster; Drew Jordahl; Xiao Zhu; Bingcan Chen; Zhongyu Yang

STAR Protocols (Sep 2021)

Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Yanxiong Pan,
Hui Li,
Qiaobin Li,
Mary Lenertz,
Isabelle Schuster,
Drew Jordahl,
Xiao Zhu,
Bingcan Chen,
Zhongyu Yang

Affiliations

Yanxiong Pan: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58102, USA
Hui Li: Department of Plant Sciences, North Dakota State University, Fargo, ND 58102, USA
Qiaobin Li: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58102, USA
Mary Lenertz: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58102, USA
Isabelle Schuster: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58102, USA
Drew Jordahl: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58102, USA
Xiao Zhu: Research Computing, Information Technology at Purdue (ITaP), Purdue University, West Lafayette, IN 47907, USA; Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA; Corresponding author
Bingcan Chen: Department of Plant Sciences, North Dakota State University, Fargo, ND 58102, USA
Zhongyu Yang: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58102, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 3
p. 100676

Abstract

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Summary: Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes.For complete information on the generation and use of this protocol, please refer to Pan et al. (2021a).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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