A soybean NAC homolog contributes to resistance to Phytophthora sojae mediated by dirigent proteins
Guolong Yu,
Jianan Zou,
Jinhui Wang,
Rongsheng Zhu,
Zhaoming Qi,
Hongwei Jiang,
Zhenbang Hu,
Mingliang Yang,
Ying Zhao,
Xiaoxia Wu,
Chunyan Liu,
Candong Li,
Xue Yang,
Zhendong Zhu,
Qingshan Chen,
Yongfu Fu,
Dawei Xin
Affiliations
Guolong Yu
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
Jianan Zou
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Jinhui Wang
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Rongsheng Zhu
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Zhaoming Qi
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Hongwei Jiang
Soybean Research Institute, Jilin Academy of Agricultural Science, Changchun 130033, Jilin, China
Zhenbang Hu
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Mingliang Yang
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Ying Zhao
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Xiaoxia Wu
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Chunyan Liu
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Candong Li
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China; Jiamusi Branch Academy of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154007, Heilongjiang, China
Xue Yang
Collaboration Centre to Russia of Institute of Grass Industry, Heilongjiang Academy of Agricultural Sciences, Harbin 150000, Heilongjiang, China
Zhendong Zhu
MOA Key Lab of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.
Qingshan Chen
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China; Corresponding authors.
Yongfu Fu
MOA Key Lab of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Dawei Xin
Key Laboratory of Soybean Biology of MOE, Key Laboratory of Soybean Biology and Breeding/Genetics of MOA, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China; Corresponding authors.
Phytophthora sojae infection severely impairs soybean production. We previously identified a dirigent protein, GmDRR1 (Glycine max Disease Resistant Response 1), that increases soybean resistance to P. sojae. However, the molecular basis of GmDRR1 function remained largely uncharacterized. In the present study, analysis of GmDRR1-RNAi, GmDRR1-overexpressing, and CRISPR/Cas9-derived Gmdrr1 mutant lines revealed that GmDRR1 expression significantly restricted P. sojae growth. Combining co-immunoprecipitation with liquid chromatography–tandem mass spectrometry revealed a GmDRR1-interacting protein, GmDRR2, which is homologous to GmDRR1. An E-coniferyl alcohol coupling assay indicated that GmDRR1 promotes the synthesis of (+)-pinoresinol, which helps to protect plants from P. sojae. The GmNAC1 (Glyma.05G025500) transcription factor bound to the GmDRR1 promoter both in vitro and in vivo to upregulate GmDRR1 expression. Soybean resistance to P. sojae was increased by overexpression of GmNAC1. Our findings suggest a novel signaling pathway involving a NAC transcription factor that mediates soybean resistance to P. sojae. Specifically, GmNAC1 directly induces GmDRR1 expression to increase resistance of soybean plants to P. sojae.
