Transcriptome sequencing reveals the potential mechanisms of dietary lutein regulation on chicken leg muscle development

Tuanhui Ren; Meng Xu; Wujian Lin; Wen Luo; Xiquan Zhang

Poultry Science (Dec 2024)

Transcriptome sequencing reveals the potential mechanisms of dietary lutein regulation on chicken leg muscle development

Tuanhui Ren,
Meng Xu,
Wujian Lin,
Wen Luo,
Xiquan Zhang

Affiliations

Tuanhui Ren: Department of Animal Genetics, College of Animal Science, Breeding and Reproduction, South China Agricultural University, Guangzhou, 510642, China; Guangdong Key Laboratory of Genome and Molecular Breeding of Agricultural Animals and Key Laboratory of Chicken Genetic Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China
Meng Xu: State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China
Wujian Lin: Department of Animal Genetics, College of Animal Science, Breeding and Reproduction, South China Agricultural University, Guangzhou, 510642, China; Guangdong Key Laboratory of Genome and Molecular Breeding of Agricultural Animals and Key Laboratory of Chicken Genetic Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China
Wen Luo: Department of Animal Genetics, College of Animal Science, Breeding and Reproduction, South China Agricultural University, Guangzhou, 510642, China; Guangdong Key Laboratory of Genome and Molecular Breeding of Agricultural Animals and Key Laboratory of Chicken Genetic Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China
Xiquan Zhang: Department of Animal Genetics, College of Animal Science, Breeding and Reproduction, South China Agricultural University, Guangzhou, 510642, China; Guangdong Key Laboratory of Genome and Molecular Breeding of Agricultural Animals and Key Laboratory of Chicken Genetic Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China; Corresponding author:

Journal volume & issue: Vol. 103, no. 12
p. 104265

Abstract

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ABSTRACT: Lutein is an antioxidant that can indicate the oxidative status of organisms through its coloration and may be involved in the development process of chicken skeletal muscle. In this study, after feeding Nanhai Yellow Chickens with lutein-containing feed for 21 d, the lutein group significantly increased the muscle fiber diameter and decreased the fiber density in the chicken's leg muscles compared to the control group. To elucidate the potential regulatory mechanisms by which lutein is involved in muscle development, RNA-seq was used to detect changes in gene expression in chicken leg muscle tissue. After data analysis, a total of 249 significantly differentially expressed genes (DEG) were identified, including TGF-β superfamily (MSTN and TGFB1) and nonreceptor tyrosine kinase c-Src (SRC). Results from GO and KEGG analysis showed that the DEGs were enriched in GO terms such as positive regulation of the ERK1/ERK2 cascade and negative regulation of myoblast differentiation, as well as signaling pathways including the Toll-like receptor signaling pathway and the MAPK signaling pathway. These significantly enriched GO terms and pathways are closely related to muscle development, suggesting that lutein may play an important role in the process of chicken muscle development. This study provides insights into the regulatory mechanisms of dietary lutein on chicken muscle development.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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