DNA repair by Rad52 liquid droplets

Roxanne Oshidari; Richard Huang; Maryam Medghalchi; Elizabeth Y. W. Tse; Nasser Ashgriz; Hyun O. Lee; Haley Wyatt; Karim Mekhail

doi:10.1038/s41467-020-14546-z

Nature Communications (Feb 2020)

DNA repair by Rad52 liquid droplets

Roxanne Oshidari,
Richard Huang,
Maryam Medghalchi,
Elizabeth Y. W. Tse,
Nasser Ashgriz,
Hyun O. Lee,
Haley Wyatt,
Karim Mekhail

Affiliations

Roxanne Oshidari: Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto
Richard Huang: Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto
Maryam Medghalchi: Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto
Elizabeth Y. W. Tse: Department of Biochemistry, Faculty of Medicine, University of Toronto
Nasser Ashgriz: Multiphase Flow and Phase Systems Laboratory, Department of Mechanical and Industrial Engineering, Faculty of Applied Science and Engineering, University of Toronto
Hyun O. Lee: Department of Biochemistry, Faculty of Medicine, University of Toronto
Haley Wyatt: Department of Biochemistry, Faculty of Medicine, University of Toronto
Karim Mekhail: Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto

DOI: https://doi.org/10.1038/s41467-020-14546-z
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 8

Abstract

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Genome dynamics allow cells to repair DNA double-strand breaks (DSBs), which are highly toxic DNA lesions. Here the authors reveal that in S. cerevisiae, Rad52 DNA repair proteins assemble in liquid droplets that work with dynamic nuclear microtubules to relocalize lesions to the nuclear periphery for repair.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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