Construction and application of M1 iron ion fluorescence system based on naphthalimide fluorescence probe
Yan Lang,
Huili Liu,
Chunbin Zhang,
Kuilin Lv
Affiliations
Yan Lang
Department of Rehabilitation Therapy, Wuyi University, Nanping, Fujian Province 354301, China
Huili Liu
Department of Medical Technology, Zhang Zhou Health Vocational College, /Collaborative Innovation Center for Translation Medical Testing and Application Technology, Zhangzhou, Fujian Province 363000, China
Chunbin Zhang
Department of Medical Technology, Zhang Zhou Health Vocational College, /Collaborative Innovation Center for Translation Medical Testing and Application Technology, Zhangzhou, Fujian Province 363000, China
Kuilin Lv
State Key Laboratory of Green Building Materials, China State Building Materials Research Institute Co., Ltd., Beijing 100000, China
In this paper, the naphthalimide fluorescence probe M1 with Fe3+ fluorescence system was successfully constructed, and its UV–visible absorption spectrum and fluorescence emission spectrum were investigated. Under optimal experimental conditions, the system has good anti-interference ability. The M1 probe has good linearity with Fe3+ concentration (1 × 10−6–15 × 10−6 mol·L−1). The detection limit reaches 7.55 × 10−8 mol·L−1. The complexation ratio of M1 probe to Fe3+ is 1:1. For the first time, we used the constructed M1 probe with Fe3+ fluorescence system to determine Fe3+ concentration in the Chinese herbal medicines, Panacis Quinquefolii Radix and Paeoniae Radix Alba, and in the fluorescence imaging of HeLa cells.
