Detection of Tomato Ringspot Virus Based on Microfluidic Impedance Sensor

Abstract

A microfluidic impedance sensor embedded with gold interdigitated array microelectrodes was utilized to rapidly detect Tomato Ringspot Virus (ToRSV) and achieve efficient and precise detection. The electrochemical impedance spectrum was obtained by immobilizing ToRSV antibody on the surface of a gold interdigital array microelectrode and mixing it with ToRSV to generate an impedance change. The electrochemical impedance spectrum was obtained. The equivalent circuit was established to analyze the mechanism of impedance change, and the quantitative linear relationship between ToRSV concentration and impedance was established. According to an equivalent circuit analysis, ToRSV increases the solution resistance Rs, the electron transfer resistance Ret on the electrode surface, and the double layer capacitance Cdl, resulting in an increase in impedance. The results reveal that the ToRSV concentration detected in the range of 0.001 to 10 μg/mL ranges from 248.8 to 687.2 kΩ at the ideal detection frequency of 10.7 Hz, with a good linear connection, R2 = 0.98. When this method’s detection limit is tested, the impedance value is 367.68 kΩ. 0.0032 μg/mL was the detection limit. The sensor is quick and easy to use, has high detection sensitivity, and can be used to detect other plant viruses.

Keywords

• microfluidic chip
• impedance sensor
• tomato ringspot virus
• quantitative detection