GE11-antigen-loaded hepatitis B virus core antigen virus-like particles efficiently bind to TNBC tumor

Long Zhang; Long Zhang; Lin Tang; Yongsheng Jiang; Yongsheng Jiang; Chenou Wang; Lijiang Huang; Lijiang Huang; Ting Ding; Tinghong Zhang; Huaqiong Li; Huaqiong Li; Longteng Xie

doi:10.3389/fonc.2023.1110751

Frontiers in Oncology (Mar 2023)

GE11-antigen-loaded hepatitis B virus core antigen virus-like particles efficiently bind to TNBC tumor

Long Zhang,
Long Zhang,
Lin Tang,
Yongsheng Jiang,
Yongsheng Jiang,
Chenou Wang,
Lijiang Huang,
Lijiang Huang,
Ting Ding,
Tinghong Zhang,
Huaqiong Li,
Huaqiong Li,
Longteng Xie

Affiliations

Long Zhang: Department of Infectious Diseases, The Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo, Zhejiang, China
Long Zhang: Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China
Lin Tang: School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, China
Yongsheng Jiang: Department of Infectious Diseases, The Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo, Zhejiang, China
Yongsheng Jiang: Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China
Chenou Wang: School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, China
Lijiang Huang: Department of Infectious Diseases, The Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo, Zhejiang, China
Lijiang Huang: Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China
Ting Ding: Department of Infectious Diseases, The Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo, Zhejiang, China
Tinghong Zhang: Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China
Huaqiong Li: Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China
Huaqiong Li: School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, China
Longteng Xie: Department of Infectious Diseases, The Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo, Zhejiang, China

DOI: https://doi.org/10.3389/fonc.2023.1110751
Journal volume & issue: Vol. 13

Abstract

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PurposeThis study aimed to explore the possibility of utilizing hepatitis B core protein (HBc) virus-like particles (VLPs) encapsulate doxorubicin (Dox) to reduce the adverse effect caused by its off-target and toxic side effect.MethodsHere, a triple-negative breast cancer (TNBC) tumor-targeting GE11-HBc VLP was constructed through genetic engineering. The GE11 peptide, a 12-amino-acid peptide targeting epidermal growth factor receptor (EGFR), was inserted into the surface protein loops of VLPs. The Dox was loaded into HBc VLPs by a thermal-triggered encapsulation strategy. The in vitro release, cytotoxicity, and cellular uptake of TNBC tumor-targeting GE11-HBc VLPs was then evaluated.ResultsThese VLPs possessed excellent stability, DOX loading efficiency, and preferentially released drug payload at high GSH levels. The insertion of GE11 targeting peptide caused improved cellular uptake and enhanced cell viability inhibitory in EGFR high-expressed TNBC cells.ConclusionTogether, these results highlight DOX-loaded, EGFR-targeted VLPs as a potentially useful therapeutic choice for EGFR-overexpressing TNBC.

Published in Frontiers in Oncology

ISSN: 2234-943X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.frontiersin.org/journals/oncology/

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