Nature Communications (Sep 2017)
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
- Tobias Weinert,
- Natacha Olieric,
- Robert Cheng,
- Steffen Brünle,
- Daniel James,
- Dmitry Ozerov,
- Dardan Gashi,
- Laura Vera,
- May Marsh,
- Kathrin Jaeger,
- Florian Dworkowski,
- Ezequiel Panepucci,
- Shibom Basu,
- Petr Skopintsev,
- Andrew S. Doré,
- Tian Geng,
- Robert M. Cooke,
- Mengning Liang,
- Andrea E. Prota,
- Valerie Panneels,
- Przemyslaw Nogly,
- Ulrich Ermler,
- Gebhard Schertler,
- Michael Hennig,
- Michel O. Steinmetz,
- Meitian Wang,
- Jörg Standfuss
Affiliations
- Tobias Weinert
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Natacha Olieric
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Robert Cheng
- LeadXpro AG
- Steffen Brünle
- Molecular Membrane Biology, Max-Planck Institute of Biophysics
- Daniel James
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Dmitry Ozerov
- Science IT, Paul Scherrer Institut
- Dardan Gashi
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Laura Vera
- Macromolecular Crystallography, Swiss Light Source, Paul Scherrer Institut
- May Marsh
- Macromolecular Crystallography, Swiss Light Source, Paul Scherrer Institut
- Kathrin Jaeger
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Florian Dworkowski
- Macromolecular Crystallography, Swiss Light Source, Paul Scherrer Institut
- Ezequiel Panepucci
- Macromolecular Crystallography, Swiss Light Source, Paul Scherrer Institut
- Shibom Basu
- Macromolecular Crystallography, Swiss Light Source, Paul Scherrer Institut
- Petr Skopintsev
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Andrew S. Doré
- Heptares Therapeutics Ltd
- Tian Geng
- Heptares Therapeutics Ltd
- Robert M. Cooke
- Heptares Therapeutics Ltd
- Mengning Liang
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Andrea E. Prota
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Valerie Panneels
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Przemyslaw Nogly
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Ulrich Ermler
- Molecular Membrane Biology, Max-Planck Institute of Biophysics
- Gebhard Schertler
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Michael Hennig
- LeadXpro AG
- Michel O. Steinmetz
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- Meitian Wang
- Macromolecular Crystallography, Swiss Light Source, Paul Scherrer Institut
- Jörg Standfuss
- Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut
- DOI
- https://doi.org/10.1038/s41467-017-00630-4
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 11
Abstract
Serial crystallography was developed for protein crystal data collection with X-ray free-electron lasers. Here the authors present several examples which show that serial crystallography using high-viscosity injectors can also be routinely employed for room-temperature data collection at synchrotrons.
