Nature Communications (May 2022)
Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain
- Sruti Rayaprolu,
- Sara Bitarafan,
- Juliet V. Santiago,
- Ranjita Betarbet,
- Sydney Sunna,
- Lihong Cheng,
- Hailian Xiao,
- Ruth S. Nelson,
- Prateek Kumar,
- Pritha Bagchi,
- Duc M. Duong,
- Annie M. Goettemoeller,
- Viktor János Oláh,
- Matt Rowan,
- Allan I. Levey,
- Levi B. Wood,
- Nicholas T. Seyfried,
- Srikant Rangaraju
Affiliations
- Sruti Rayaprolu
- Department of Neurology, Emory University
- Sara Bitarafan
- Georgia W. Woodruff School of Mechanical Engineering, Parker H. Petit Institute for Bioengineering and Bioscience, and Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology
- Juliet V. Santiago
- Department of Neurology, Emory University
- Ranjita Betarbet
- Department of Neurology, Emory University
- Sydney Sunna
- Department of Neurology, Emory University
- Lihong Cheng
- Department of Neurology, Emory University
- Hailian Xiao
- Department of Neurology, Emory University
- Ruth S. Nelson
- Department of Neurology, Emory University
- Prateek Kumar
- Department of Neurology, Emory University
- Pritha Bagchi
- Center for Neurodegenerative Diseases, Emory University
- Duc M. Duong
- Center for Neurodegenerative Diseases, Emory University
- Annie M. Goettemoeller
- Department of Cell Biology, Emory University
- Viktor János Oláh
- Department of Cell Biology, Emory University
- Matt Rowan
- Department of Cell Biology, Emory University
- Allan I. Levey
- Department of Neurology, Emory University
- Levi B. Wood
- Georgia W. Woodruff School of Mechanical Engineering, Parker H. Petit Institute for Bioengineering and Bioscience, and Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology
- Nicholas T. Seyfried
- Center for Neurodegenerative Diseases, Emory University
- Srikant Rangaraju
- Department of Neurology, Emory University
- DOI
- https://doi.org/10.1038/s41467-022-30623-x
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 18
Abstract
Current isolation-based approaches for cell type-specific proteomics pose several challenges. Here, the authors present an approach for in vivo cell type-specific protein labeling to characterize proteomic differences between neurons and astrocytes in their native state in adult mouse brain.
