Combinatory Effect of Pequi Oil (<i>Caryocar brasiliense</i>)-Based Nanoemulsions Associated to Docetaxel and Anacardic Acid (<i>Anacardium occidentale</i>) in Triple-Negative Breast Cancer Cells In Vitro
Alicia Simalie Ombredane,
Natália Ornelas Martins,
Gabriela Mara Vieira de Souza,
Victor Hugo Sousa Araujo,
Ísis O. Szlachetka,
Sebastião William da Silva,
Márcia Cristina Oliveira da Rocha,
Andressa Souza de Oliveira,
Cleonice Andrade Holanda,
Luiz Antonio Soares Romeiro,
Elysa Beatriz de Oliveira Damas,
Ricardo Bentes Azevedo,
Graziella Anselmo Joanitti
Affiliations
Alicia Simalie Ombredane
Laboratory of Bioactive Compounds and Nanobiotechnology (LCBNano), University of Brasilia, Campus Universitário—Centro Metropolitano, Ceilandia Sul, Brasilia 72220-275, Brazil
Natália Ornelas Martins
Laboratory of Bioactive Compounds and Nanobiotechnology (LCBNano), University of Brasilia, Campus Universitário—Centro Metropolitano, Ceilandia Sul, Brasilia 72220-275, Brazil
Gabriela Mara Vieira de Souza
Laboratory of Bioactive Compounds and Nanobiotechnology (LCBNano), University of Brasilia, Campus Universitário—Centro Metropolitano, Ceilandia Sul, Brasilia 72220-275, Brazil
Victor Hugo Sousa Araujo
Laboratory of Bioactive Compounds and Nanobiotechnology (LCBNano), University of Brasilia, Campus Universitário—Centro Metropolitano, Ceilandia Sul, Brasilia 72220-275, Brazil
Ísis O. Szlachetka
Post-Graduation Program in Nanoscience and Nanobiotechnology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasilia 70910-900, Brazil
Sebastião William da Silva
Post-Graduation Program in Nanoscience and Nanobiotechnology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasilia 70910-900, Brazil
Márcia Cristina Oliveira da Rocha
Post-Graduation Program in Nanoscience and Nanobiotechnology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasilia 70910-900, Brazil
Andressa Souza de Oliveira
Graduate Program in Pharmaceutical Sciences, Department of Pharmacy, Health Sciences Faculty, University of Brasilia, Brasilia 70910-900, Brazil
Cleonice Andrade Holanda
Graduate Program in Pharmaceutical Sciences, Department of Pharmacy, Health Sciences Faculty, University of Brasilia, Brasilia 70910-900, Brazil
Luiz Antonio Soares Romeiro
Graduate Program in Pharmaceutical Sciences, Department of Pharmacy, Health Sciences Faculty, University of Brasilia, Brasilia 70910-900, Brazil
Elysa Beatriz de Oliveira Damas
Laboratory of Bioactive Compounds and Nanobiotechnology (LCBNano), University of Brasilia, Campus Universitário—Centro Metropolitano, Ceilandia Sul, Brasilia 72220-275, Brazil
Ricardo Bentes Azevedo
Post-Graduation Program in Nanoscience and Nanobiotechnology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasilia 70910-900, Brazil
Graziella Anselmo Joanitti
Laboratory of Bioactive Compounds and Nanobiotechnology (LCBNano), University of Brasilia, Campus Universitário—Centro Metropolitano, Ceilandia Sul, Brasilia 72220-275, Brazil
Combination therapy integrated with nanotechnology offers a promising alternative for breast cancer treatment. The inclusion of pequi oil, anacardic acid (AA), and docetaxel (DTX) in a nanoemulsion can amplify the antitumor effects of each molecule while reducing adverse effects. Therefore, the study aims to develop pequi oil-based nanoemulsions (PeNE) containing DTX (PDTX) or AA (PAA) and to evaluate their cytotoxicity against triple-negative breast cancer cells (4T1) in vitro. The PeNE without and with AA (PAA) and DTX (PDTX) were prepared by sonication and characterized by ZetaSizer® and electronic transmission microscopy. Viability testing and combination index (CI) were determined by MTT and Chou-Talalay methods, respectively. Flow cytometry was employed to investigate the effects of the formulations on cell structures. PeNE, PDTX, and PAA showed hydrodynamic diameter p 1). Additionally, PDTX + PAA induced an expressive morphological change, a major change in lysosome membrane permeation and mitochondria membrane permeation, cell cycle blockage in G2/M, and phosphatidylserine exposure. The study highlights the successful use of pequi oil nanoemulsions as delivery systems for DTX and AA, which enhances their antitumor effects against breast cancer cells. This nanotechnological approach shows significant potential for the treatment of triple-negative breast cancer.
