Establishment of an induced pluripotent stem cell line (ICGi026-A) from peripheral blood mononuclear cells of a patient with fragile X syndrome

M.M. Gridina; P.A. Orlova; J.M. Minina; E.M. Shitik; N.A. Lemskaya; I.V. Grishchenko; A.A. Dolskiy; A.R. Shorina; Y.V. Maksimova; D.V. Yudkin; O.L. Serov

Stem Cell Research (Dec 2020)

Establishment of an induced pluripotent stem cell line (ICGi026-A) from peripheral blood mononuclear cells of a patient with fragile X syndrome

M.M. Gridina,
P.A. Orlova,
J.M. Minina,
E.M. Shitik,
N.A. Lemskaya,
I.V. Grishchenko,
A.A. Dolskiy,
A.R. Shorina,
Y.V. Maksimova,
D.V. Yudkin,
O.L. Serov

Affiliations

M.M. Gridina: Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia; Corresponding author.
P.A. Orlova: Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
J.M. Minina: Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
E.M. Shitik: State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo, Novosibirsk Region, Russia
N.A. Lemskaya: State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo, Novosibirsk Region, Russia; Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, Russia
I.V. Grishchenko: State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo, Novosibirsk Region, Russia
A.A. Dolskiy: SBIH Novosibirsk Municipal Clinical Hospital №1, Novosibirsk, Russia
A.R. Shorina: SBIH Novosibirsk Municipal Clinical Hospital №1, Novosibirsk, Russia
Y.V. Maksimova: SBIH Novosibirsk Municipal Clinical Hospital №1, Novosibirsk, Russia; Novosibirsk State Medical University, Novosibirsk, Russia
D.V. Yudkin: State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo, Novosibirsk Region, Russia
O.L. Serov: Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia

Journal volume & issue: Vol. 49
p. 102070

Abstract

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Expansion over 200 CGG repeats in FMR1 gene causes inherited intellectual disability or autism spectrum disorder named as fragile X syndrome. Despite the known cause fragile X syndrome pathogenesis has not been specified yet. The ICGi026-A iPSCs line was obtained by the reprogramming of the peripheral blood mononuclear cells from a 9-year-old boy with fragile X syndrome. The ICGi026-A iPSCs expressed pluripotency markers, had a normal male karyotype (46, XY) and had the capacity to in vivo differentiate into the cells of three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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