Frontiers in Microbiology (Jul 2024)
Isolation and identification of a novel porcine-related recombinant mammalian orthoreovirus type 3 strain from cattle in Guangxi Province, China
- Yuhang Luo,
- Yuhang Luo,
- Yuhang Luo,
- Yuhang Luo,
- Yanglin Wang,
- Yanglin Wang,
- Yanglin Wang,
- Wenfei Tang,
- Wenfei Tang,
- Wenfei Tang,
- Cui Wang,
- Cui Wang,
- Cui Wang,
- Cui Wang,
- Huanghao Liu,
- Huanghao Liu,
- Huanghao Liu,
- Xiaoling Wang,
- Jiang Xie,
- Jie Wang,
- Jie Wang,
- Jie Wang,
- Kang Ouyang,
- Kang Ouyang,
- Kang Ouyang,
- Ying Chen,
- Ying Chen,
- Ying Chen,
- Zuzhang Wei,
- Zuzhang Wei,
- Zuzhang Wei,
- Yifeng Qin,
- Yifeng Qin,
- Yifeng Qin,
- Yan Pan,
- Weijian Huang,
- Weijian Huang,
- Weijian Huang
Affiliations
- Yuhang Luo
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Yuhang Luo
- Guangxi Vocational University of Agriculture, Nanning, China
- Yuhang Luo
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Yuhang Luo
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Yanglin Wang
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Yanglin Wang
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Yanglin Wang
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Wenfei Tang
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Wenfei Tang
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Wenfei Tang
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Cui Wang
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Cui Wang
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Cui Wang
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Cui Wang
- Liuzhou Center for Animal Disease Control and Prevention, Liuzhou, China
- Huanghao Liu
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Huanghao Liu
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Huanghao Liu
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Xiaoling Wang
- Guangxi Vocational University of Agriculture, Nanning, China
- Jiang Xie
- Guangxi Vocational University of Agriculture, Nanning, China
- Jie Wang
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Jie Wang
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Jie Wang
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Kang Ouyang
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Kang Ouyang
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Kang Ouyang
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Ying Chen
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Ying Chen
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Ying Chen
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Zuzhang Wei
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Zuzhang Wei
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Zuzhang Wei
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Yifeng Qin
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Yifeng Qin
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Yifeng Qin
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- Yan Pan
- Guangxi Vocational University of Agriculture, Nanning, China
- Weijian Huang
- Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China
- Weijian Huang
- Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China
- Weijian Huang
- Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, China
- DOI
- https://doi.org/10.3389/fmicb.2024.1419691
- Journal volume & issue
-
Vol. 15
Abstract
The Mammalian orthoreovirus (MRV) infects various mammals, including humans, and is linked to gastrointestinal, respiratory, and neurological diseases. A recent outbreak in Liuzhou, Guangxi, China, led to the isolation of a new MRV strain, GXLZ2301, from fecal samples. This strain replicates in multiple cell lines and forms lattice-like structures. Infected cells exhibit single-cell death and syncytia formation. The virus’s titers peaked at 107.2 TCID50/0.1 mL in PK-15 and BHK cells, with the lowest at 103.88 TCID50/0.1 mL in A549 cells. Electron microscopy showed no envelope with a diameter of about 70 nm. Genetic analysis revealed GXLZ2301 as a recombinant strain with gene segments from humans, cows, and pigs, similar to type 3 MRV strains from Italy (2015-2016). Pathogenicity tests indicated that while the bovine MRV strain did not cause clinical symptoms in mice, it caused significant damage to the gut, lungs, liver, kidneys, and brain. The emergence of this MRV strain may pose a threat to the health of animals and humans, and it is recommended that its epidemiology and recombination be closely monitored.
Keywords
