Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

K. K. Khoo; I. Galleano; F. Gasparri; R. Wieneke; H. Harms; M. H. Poulsen; H. C. Chua; M. Wulf; R. Tampé; S. A. Pless

doi:10.1038/s41467-020-16208-6

Nature Communications (May 2020)

Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

K. K. Khoo,
I. Galleano,
F. Gasparri,
R. Wieneke,
H. Harms,
M. H. Poulsen,
H. C. Chua,
M. Wulf,
R. Tampé,
S. A. Pless

Affiliations

K. K. Khoo: Department of Drug Design and Pharmacology, University of Copenhagen
I. Galleano: Department of Drug Design and Pharmacology, University of Copenhagen
F. Gasparri: Department of Drug Design and Pharmacology, University of Copenhagen
R. Wieneke: Institute of Biochemistry, Biocenter, Goethe University Frankfurt
H. Harms: Department of Drug Design and Pharmacology, University of Copenhagen
M. H. Poulsen: Department of Drug Design and Pharmacology, University of Copenhagen
H. C. Chua: Department of Drug Design and Pharmacology, University of Copenhagen
M. Wulf: Department of Drug Design and Pharmacology, University of Copenhagen
R. Tampé: Institute of Biochemistry, Biocenter, Goethe University Frankfurt
S. A. Pless: Department of Drug Design and Pharmacology, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-020-16208-6
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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Chemical modification of proteins can be used to decipher function or use that function for therapeutic purposes. Here, the authors insert synthetic peptides via tandem protein trans-splicing to add post-translational modifications or non-canonical amino acids.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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