The relationship between endoplasmic reticulum stress and apoptosis in the process of adipose-derived stromal cells differentiating into astrocytes

Abstract

The potential of adult adipose-derived stromal cells (ADSCs) to differentiate into astrocytes holds promise for future cell transplantation therapies. However, the growth of differentiated astrocytes is unstable, and their survival rate is low. Endoplasmic reticulum (ER) pathway mediated apoptosis is one of the causes of cell death, but whether there is ER stress response in the differentiation of ADSCs into astrocytes is still unclear. In this study, the expression of protein factors related to endoplasmic reticulum stress (ERS) and apoptosis, including GRP78, ATF6, PERK, CHOP, Caspase12, and Caspase3, was detected in cells. It was found that the expression of ERS pro-survival factors was highest in the ADSCs group and decreased with prolonged induction time. Conversely, the expression levels of pro-apoptotic factors increased with the extension of induction time. Thus, ERS occurs during the differentiation of ADSCs into astrocytes, and ERS can mediate apoptosis of ADSC-derived astrocytes.

Keywords

• Adipose-derived stromal cells
• apoptosis
• astrocytes
• endoplasmic reticulum stress
• induction