Ionically Imprinting-Based Copper (II) Label-Free Detection for Preventing Hearing Loss
Huan Wang,
Hui Zhang,
Xiaoli Zhang,
Hong Chen,
Ling Lu,
Renjie Chai
Affiliations
Huan Wang
The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518033, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China; Corresponding authors.
Hui Zhang
State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital & Advanced Institute for Life and Health, Southeast University, Nanjing 210096, China
Xiaoli Zhang
Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Hong Chen
Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Ling Lu
Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China; Corresponding authors.
Renjie Chai
State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital & Advanced Institute for Life and Health, Southeast University, Nanjing 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China; Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China; Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing 100101, China; Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China; Corresponding authors.
Copper is a microelement with important physiological functions in the body. However, the excess copper ion (Cu2+) may cause severe health problems, such as hair cell apoptosis and the resultant hearing loss. Therefore, the assay of Cu2+ is important. We integrate ionic imprinting technology (IIT) and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads (IIHBs) as a low-cost and high-specificity platform for Cu2+ detection. The IIHBs have a macroporous microstructure, uniform size, vivid structural color, and magnetic responsiveness. When incubated in solution, IIHBs recognize Cu2+ and exhibit a reflective peak change, thereby achieving label-free detection. In addition, benefiting from the IIT, the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100 μmol·L−1. These features indicated that the developed IIHBs are promising candidates for Cu2+ detection, particularly for the prevention of hearing loss.
