Ionically Imprinting-Based Copper (II) Label-Free Detection for Preventing Hearing Loss

Huan Wang; Hui Zhang; Xiaoli Zhang; Hong Chen; Ling Lu; Renjie Chai

Engineering (Feb 2024)

Ionically Imprinting-Based Copper (II) Label-Free Detection for Preventing Hearing Loss

Huan Wang,
Hui Zhang,
Xiaoli Zhang,
Hong Chen,
Ling Lu,
Renjie Chai

Affiliations

Huan Wang: The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518033, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China; Corresponding authors.
Hui Zhang: State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital & Advanced Institute for Life and Health, Southeast University, Nanjing 210096, China
Xiaoli Zhang: Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Hong Chen: Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Ling Lu: Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China; Corresponding authors.
Renjie Chai: State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital & Advanced Institute for Life and Health, Southeast University, Nanjing 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China; Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China; Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing 100101, China; Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China; Corresponding authors.

Journal volume & issue: Vol. 33
pp. 276 – 282

Abstract

Read online

Copper is a microelement with important physiological functions in the body. However, the excess copper ion (Cu2+) may cause severe health problems, such as hair cell apoptosis and the resultant hearing loss. Therefore, the assay of Cu2+ is important. We integrate ionic imprinting technology (IIT) and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads (IIHBs) as a low-cost and high-specificity platform for Cu2+ detection. The IIHBs have a macroporous microstructure, uniform size, vivid structural color, and magnetic responsiveness. When incubated in solution, IIHBs recognize Cu2+ and exhibit a reflective peak change, thereby achieving label-free detection. In addition, benefiting from the IIT, the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100 μmol·L−1. These features indicated that the developed IIHBs are promising candidates for Cu2+ detection, particularly for the prevention of hearing loss.

Published in Engineering

ISSN: 2095-8099 (Print); 2096-0026 (Online)
Publisher: Elsevier
Country of publisher: China
LCC subjects: Technology: Engineering (General). Civil engineering (General)
Website: http://www.journals.elsevier.com/engineering

About the journal

Abstract

Keywords