Morphological Evaluation and Immunohistochemical Analysis of the Reparative Potential of the Buccal Fat Pad
Roman Zhidkov,
Andrew Panin,
Aleksei Drobyshev,
Tatiana Demura,
Sofya Avraamova,
Petr Aleksandrov,
Anastasia Kolesnikova,
Hadi Darawsheh,
Anna Turkina,
Nicolai Redko,
Yaroslav Skakunov,
Elena Karpova,
Anzhela Brago,
Aleksandr Tsitsiashvili,
Yuriy Vasil’ev
Affiliations
Roman Zhidkov
Federal State Budgetary Educational Institution of Higher Education «ROSUNIMED» of the Ministry of Health of the Russian Federation, 119991 Moscow, Russia
Andrew Panin
Federal State Budgetary Educational Institution of Higher Education «ROSUNIMED» of the Ministry of Health of the Russian Federation, 119991 Moscow, Russia
Aleksei Drobyshev
Federal State Budgetary Educational Institution of Higher Education «ROSUNIMED» of the Ministry of Health of the Russian Federation, 119991 Moscow, Russia
Tatiana Demura
Institute of Clinical Morphology and Digital Pathology, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia
Sofya Avraamova
Institute of Clinical Morphology and Digital Pathology, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia
Petr Aleksandrov
Institute of Clinical Morphology and Digital Pathology, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia
Anastasia Kolesnikova
Institute of Clinical Morphology and Digital Pathology, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia
Hadi Darawsheh
N.V. Sklifosovskiy Institute of Clinical Medicine, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia
Anna Turkina
E.V. Borovsky Institute of Dentistry, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia
Nicolai Redko
Federal State Budgetary Educational Institution of Higher Education «ROSUNIMED» of the Ministry of Health of the Russian Federation, 119991 Moscow, Russia
Yaroslav Skakunov
Federal State Budgetary Educational Institution of Higher Education «ROSUNIMED» of the Ministry of Health of the Russian Federation, 119991 Moscow, Russia
Elena Karpova
Federal State Budgetary Educational Institution of Higher Education, Pirogov Russian National Research Medical University of the Ministry of Health of the Russian Federation, 119991 Moscow, Russia
Anzhela Brago
Department of Propedeutics of Dental Diseases, Medical Institute, Peoples’ Friendship University of Russia Named after Patrice Lumumba, 117198 Moscow, Russia
Aleksandr Tsitsiashvili
Federal State Budgetary Educational Institution of Higher Education «ROSUNIMED» of the Ministry of Health of the Russian Federation, 119991 Moscow, Russia
Yuriy Vasil’ev
N.V. Sklifosovskiy Institute of Clinical Medicine, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia
Background and Objectives: There are many surgical techniques for oroantral communication treatment, one of which is the buccal fat pad. Of particular interest is the high reparative potential of the buccal fat pad, which may be contributed to by the presence of mesenchymal stem cells. The purpose of this work is to evaluate the reparative potential of BFP cells using morphological and immunohistochemical examination. Materials and Methods: 30 BFP samples were provided by the Clinic of Maxillofacial and Plastic Surgery of the Russian University of Medicine (Moscow, Russia) from 28 patients. Morphological examination of 30 BFP samples was performed at the Institute of Clinical Morphology and Digital Pathology of Sechenov University. Hematoxylin–eosin, Masson trichrome staining and immunohistochemical examination were performed to detect MSCs using primary antibodies CD133, CD44 and CD10. Results: During staining with hematoxylin–eosin and Masson’s trichrome, we detected adipocytes of white adipose tissue united into lobules separated by connective tissue layers, a large number of vessels of different calibers, as well as the general capsule of BFP. The thin connective tissue layers contained neurovascular bundles. Statistical processing of the results of the IHC examination of the samples using the Mann–Whitney criterion revealed that the total number of samples in which the expression of CD44, CD10 and CD133 antigens was confirmed was statistically significantly higher than the number of samples where the expression was not detected (p Conclusions: During the morphological study of the BFP samples, we revealed statistically significant signs of MSCs presence (p < 0.05), including in the brown fat tissue, which proves the high reparative potential of this type of tissue and can make the BFP a choice option among other autogenous donor materials when eliminating OAC and other surgical interventions in the maxillofacial region.
