Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR

Tao Zhang; Cong Liu; Wuping Zhou; Keming Jiang; Chenyu Yin; Cong Liu; Zhiqiang Zhang; Haiwen Li

doi:10.1155/2019/3528345

Journal of Analytical Methods in Chemistry (Jan 2019)

Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR

Tao Zhang,
Cong Liu,
Wuping Zhou,
Keming Jiang,
Chenyu Yin,
Cong Liu,
Zhiqiang Zhang,
Haiwen Li

Affiliations

Tao Zhang: Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
Cong Liu: Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
Wuping Zhou: Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
Keming Jiang: Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
Chenyu Yin: Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
Cong Liu: Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
Zhiqiang Zhang: Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
Haiwen Li: Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China

DOI: https://doi.org/10.1155/2019/3528345
Journal volume & issue: Vol. 2019

Abstract

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In this study, an ultrasensitive detection method for aqueous Pb2+ based on digital polymerase chain reaction (dPCR) technology and a Pb2+-dependent DNAzyme was developed. In the presence of Pb2+, the Gr-5 DNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting in an increase in the amount of template DNA available for dPCR and a resultant change in the number of droplets showing a positive signal. Moreover, the detection system was found to be sensitive and stable in environmental sample detection. In summary, an ultrasensitive quantitative detection method for Pb2+ within environmental substrates was established.

Published in Journal of Analytical Methods in Chemistry

ISSN: 2090-8865 (Print); 2090-8873 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry: Analytical chemistry
Website: https://onlinelibrary.wiley.com/journal/1471

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