Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization

Juan F. Bada Juarez; Peter J. Judge; Suliman Adam; Danny Axford; Javier Vinals; James Birch; Tristan O. C. Kwan; Kin Kuan Hoi; Hsin-Yung Yen; Anthony Vial; Pierre-Emmanuel Milhiet; Carol V. Robinson; Igor Schapiro; Isabel Moraes; Anthony Watts

doi:10.1038/s41467-020-20596-0

Nature Communications (Jan 2021)

Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization

Juan F. Bada Juarez,
Peter J. Judge,
Suliman Adam,
Danny Axford,
Javier Vinals,
James Birch,
Tristan O. C. Kwan,
Kin Kuan Hoi,
Hsin-Yung Yen,
Anthony Vial,
Pierre-Emmanuel Milhiet,
Carol V. Robinson,
Igor Schapiro,
Isabel Moraes,
Anthony Watts

Affiliations

Juan F. Bada Juarez: Biochemistry Department, Oxford University, South Parks Road
Peter J. Judge: Biochemistry Department, Oxford University, South Parks Road
Suliman Adam: Fritz Haber Center for Molecular Dynamics Research, Institute of Chemistry, Hebrew University of Jerusalem
Danny Axford: Diamond Light Source, Harwell Science and Innovation Campus
Javier Vinals: Biochemistry Department, Oxford University, South Parks Road
James Birch: Diamond Light Source, Harwell Science and Innovation Campus
Tristan O. C. Kwan: Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus
Kin Kuan Hoi: Chemistry Research Laboratory, Oxford University
Hsin-Yung Yen: OMass Therapeutics, The Schrodinger Building, Oxford Science Park
Anthony Vial: Centre de Biochimie Structurale (CBS), INSERM, CNRS, University of Montpellier
Pierre-Emmanuel Milhiet: Centre de Biochimie Structurale (CBS), INSERM, CNRS, University of Montpellier
Carol V. Robinson: Chemistry Research Laboratory, Oxford University
Igor Schapiro: Fritz Haber Center for Molecular Dynamics Research, Institute of Chemistry, Hebrew University of Jerusalem
Isabel Moraes: Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus
Anthony Watts: Biochemistry Department, Oxford University, South Parks Road

DOI: https://doi.org/10.1038/s41467-020-20596-0
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 10

Abstract

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Archaerhodopsin-3 (AR3) mutants are commonly used in optogenetics for neuron silencing and membrane voltage sensing. High-resolution crystal structures show that desensitization of the AR3 photoreceptor occurs when internal hydrogen-bonded water networks are modified in response to changes in chromophore isomerization.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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