Possibility of using exometabolites Bifidobacterium bifidum № 791 in traumatology and orthopedics

Abstract

Aim. To study the effect of exometabolites of Bifidobacterium bifidum on the biological properties of causative agents of purulent-septic complications in trauma and orthopedic practice and to evaluate their effect on reparative bone tissue regeneration in the peri-implant zone in the postoperative period. Materials and methods. Using the museum cultures of B. bifidum 791, S. aureus 25923 ATCC, P. aeruginosa 27853 ATCC and antibiotic-resistant hospital strains, isolated from patients with periprosthetic infection and osteomyelitis (S.aureus 889, S. epidermidis 2041, P. aeruginosa 9672). The supernatant (exometabolites) of B. bifidum 791 was obtained from a broth culture, which was centrifuged and passed through a membrane filter. In studying the effect of exometabolites of bifidobacteria on the proliferative activity of bacteria, the two-day supernatant of B. bifidum was co-incubated for 24 cultures of museum and hospital strains. The biofilm formation in vitro experiment was studied after 24 and 96 hours. Treatment of implants with exometabolites of bifidobacteria was carried out for 30 minutes. Experimental research was carried out on rabbits of breed «Flandr». Titanium implants without coating (control) and with porous SBKFM coating treated with exometabolites of B. bifidum (experiment) were screwed into the tibial bones of animals. Histological examination and computed microtomography were performed using a SkyScan 1172 instrument (BRUKER). Result. Depending on the time of cultivation of bifidobacteria, the supernatant B. bifidum 791 had a bacteriostatic and bactericidal effect to varying degrees, and also inhibited the biofilm formation of the studied microorganism cultures, including antibiotic-resistant strains. In in vivo experiments, the use of porous SBKFM coatings on titanium implants treated with the supernatant of bifidobacteria made it possible to maintain the mineral density indices of the peri-implantation zone at a high level in comparison with the use of control titanium implants without coating. Conclusion. Positive results of preclinical experiments are promising for further clinical research in trauma and orthopedic practice, which ultimately will reduce the risk of developing septic complications and prevent the migration of metal structures and instability of endoprostheses in the postoperative period.

Keywords

• b. bifidum 791
• biofilms of microorganisms
• antibiotic-resistant strains
• traumatologic-orthopedic practice
• titanium implants