Nature Communications (Jul 2016)

Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling

  • Susanne Nichterwitz,
  • Geng Chen,
  • Julio Aguila Benitez,
  • Marlene Yilmaz,
  • Helena Storvall,
  • Ming Cao,
  • Rickard Sandberg,
  • Qiaolin Deng,
  • Eva Hedlund

DOI
https://doi.org/10.1038/ncomms12139
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 11

Abstract

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Laser capture microscopy (LCM) coupled with global transcriptome profiling requires relatively large numbers of cells. Here, the authors show that LCM coupled with full-length mRNA-sequencing (LCM-seq) can sequence single cells, and that LCM-seq can provide biological insight on highly similar neuronal populations.