Nature Communications (Jul 2016)
Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
Abstract
Laser capture microscopy (LCM) coupled with global transcriptome profiling requires relatively large numbers of cells. Here, the authors show that LCM coupled with full-length mRNA-sequencing (LCM-seq) can sequence single cells, and that LCM-seq can provide biological insight on highly similar neuronal populations.