Investigation of the Protective Effects of Magnesium on Bupivacaine-Induced Toxicity at the Level of Colon Cell Culture
Ceren Önal,
Kemal Tolga Saraçoğlu,
Ayten Saraçoğlu,
Beyza Nur Özkan,
Eray Metin Güler,
Gülten Arslan,
Seçil Azime Karakuş,
Yekbun Bulun,
Tomasz Gaszynski,
Pawel Ratajczyk
Affiliations
Ceren Önal
Department of Anesthesiology and Reanimation, Ağrı Research and Training Hospital, Ağrı 04200, Türkiye
Kemal Tolga Saraçoğlu
Department of Anaesthesiology, ICU & Perioperative Medicine, Hazm Mebaireek General Hospital HMC, Qatar University College of Medicine, Doha P.O. Box 2713, Qatar
Ayten Saraçoğlu
Department of Anaesthesiology, ICU & Perioperative Medicine, Aisha Bint Hamad Al Attiyah Hospital HMC, Qatar University College of Medicine, Doha P.O. Box 2713, Qatar
Beyza Nur Özkan
Department of Medical Biochemistry, Hamidiye Faculty of Medicine, University of Health Sciences Türkiye, İstanbul 34480, Türkiye
Eray Metin Güler
Department of Medical Biochemistry, Hamidiye Faculty of Medicine, University of Health Sciences Türkiye, İstanbul 34480, Türkiye
Gülten Arslan
Department of Anesthesiology and Reanimation, University of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, İstanbul 34865, Türkiye
Seçil Azime Karakuş
Department of Anesthesiology and Reanimation, University of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, İstanbul 34865, Türkiye
Yekbun Bulun
Department of Anesthesiology and Reanimation, Bingöl State Hospital, Bingöl 12000, Türkiye
Tomasz Gaszynski
Department of Anesthesiology and Intensive Therapy, Medical University of Lodz, 90-419 Łódź, Poland
Pawel Ratajczyk
Department of Anesthesiology and Intensive Therapy, Medical University of Lodz, 90-419 Łódź, Poland
The primary objective of this in vitro study was to prevent the risk of toxicity associated with bupivacaine, widely used in clinical practice, by using magnesium (Mg), a readily available and cost-effective element, as an adjuvant. We hypothesized that Mg might exhibit a protective effect against cytotoxicity in a colon cell culture model under conditions of bupivacaine-induced LAST. Our secondary aim was to investigate its effect on genotoxicity, apoptosis, and iROS. CCD-18Co cells were used in our study. Control group (group C), Bupivacaine group (group B), Magnesium group (group M), and Bupivacaine+Mg group (group BM) were created. The viability of CCD-18Co cells incubated for 24 h in group C was determined to be 100%. These cells were evenly divided, and bupivacaine was administered to group B at concentrations of 5 to 300 μM. In group M, doses of Mg at 0.625 to 320 mEq were added. It was determined that the maximum viability was observed at a Mg dose of 40 mEq (p p p p p p p p p p p p p p p p p pp p p p p p p < 0.05). In conclusion, Mg exhibits a protective effect against bupivacaine-induced toxicity.
