Frontiers in Microbiology (Nov 2022)
Mouse adaptation of H6 avian influenza viruses and their molecular characteristics
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Jianxi Gong,
- Jianxi Gong,
- Jianxi Gong,
- Jianxi Gong,
- Jianjun Sang,
- Wenjie Jiang,
- Wenjie Jiang,
- Wenjie Jiang,
- Wenjie Jiang,
- Zhehong Zhao,
- Zhehong Zhao,
- Zhehong Zhao,
- Zhehong Zhao,
- Mingjun Lian,
- Mingjun Lian,
- Mingjun Lian,
- Mingjun Lian,
- Ting Tang,
- Ting Tang,
- Ting Tang,
- Ting Tang,
- Yafeng Li,
- Yafeng Li,
- Yafeng Li,
- Yafeng Li,
- Qiuqi Kan,
- Qiuqi Kan,
- Qiuqi Kan,
- Qiuqi Kan,
- Quan Xie,
- Quan Xie,
- Quan Xie,
- Quan Xie,
- Tuofan Li,
- Tuofan Li,
- Tuofan Li,
- Tuofan Li,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao,
- Wei Gao,
- Wei Gao,
- Wei Gao,
- Wei Gao,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye
Affiliations
- Zhimin Wan
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Zhimin Wan
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Zhimin Wan
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Zhimin Wan
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Jianxi Gong
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Jianxi Gong
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Jianxi Gong
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Jianxi Gong
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Jianjun Sang
- Sinopharm Yangzhou VAC Biological Engineering Co. Ltd, Yangzhou, Jiangsu, China
- Wenjie Jiang
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Wenjie Jiang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Wenjie Jiang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Wenjie Jiang
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Zhehong Zhao
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Zhehong Zhao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Zhehong Zhao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Zhehong Zhao
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Mingjun Lian
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Mingjun Lian
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Mingjun Lian
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Mingjun Lian
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Ting Tang
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Ting Tang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Ting Tang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Ting Tang
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Yafeng Li
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Yafeng Li
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Yafeng Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Yafeng Li
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Qiuqi Kan
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Qiuqi Kan
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Qiuqi Kan
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Qiuqi Kan
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Quan Xie
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Quan Xie
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Quan Xie
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Quan Xie
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Tuofan Li
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Tuofan Li
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Tuofan Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Tuofan Li
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Hongxia Shao
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Hongxia Shao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Hongxia Shao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Hongxia Shao
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Wei Gao
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Wei Gao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Wei Gao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Wei Gao
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Aijian Qin
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Aijian Qin
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Aijian Qin
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Aijian Qin
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Jianqiang Ye
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China
- Jianqiang Ye
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Jianqiang Ye
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Jianqiang Ye
- Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- DOI
- https://doi.org/10.3389/fmicb.2022.1049979
- Journal volume & issue
-
Vol. 13
Abstract
H6 avian influenza viruses (AIVs) not only continue to circulate in both domestic poultry and wild waterfowl, but also have occasionally caused spillovers infections in pigs and humans, posing a potential threat to public health. However, the molecular mechanism of H6 AIV adaptation to mammals remains largely unknown. In this study, two mouse-adapted (MA) H6 AIV strains, named as MA E-Teal/417 and MA GWF-Goose/740, were generated through blind passages in BALB/c mice. The two MA H6 strains replicated more efficiently and showed higher virulence than the corresponding wild type (WT) H6 strains in mice. Genome sequencing revealed that MA E-Teal/417 and MA GWF-Goose/740 carried six amino acid mutations (PB2-T224A/E627K, HA-G124R, NA-F167L/Y356H and M1-M92R), and four amino acid mutations (PB1-K577E, PA-T97I/D514E and HA-T276K), respectively, when compared to the corresponding WT virus. Receptor binding assay showed MA E-Teal/417 had stronger binding activity to α-2,3 SA than WT E-Teal/417. Moreover, the polymerase activity analysis found the RNP polymerase activity of both MA H6 viruses was significantly higher than that of the corresponding WT virus in 293T cells. All these demonstrate that H6 AIV can acquire limit amino acid substitutions to adapt to mammals and increase virulence, highlighting the significance of monitoring such mutations of H6 AIV in the field for alarming the potential of its cross-transmission and pathogenesis in mammals.
Keywords
