Background: Meditation is a mind and body practice for increasing calmness and physical relaxation, improving psychological balance, coping with illness, and enhancing overall health and well-being. Many studies have already been carried out to see the effect of Yoga, Pranayam, meditation, Sudarhan Kriya on physiological parameters, but till date, there is not a single study which shows the cumulative effect of yoga, pranayam, meditation along with sudarshankriya on physiological parameters. Methods: This observational study was conducted in the Department of Physiology, Rajasthan University of Health Sciences, Jaipur and in association with Art of living organization. The study participants were enrolled into three groups. In the first group, 10 participants of Advance Meditation Program (AMP), who were doing this program for the first time, in second group, 10 participants who were doing AMP along with regularly practicing Padam Sadhna for at least 2 years and finally, in the third group, 10 participants who were not practicing any kind of meditation or yoga. After consent, subjects filled the Cohen's stress questionnaire. Electrocardiography (ECG) was recorded before AMP and after AMP. Results: There was significant difference in heart rate (P = 0.002), systolic blood pressure (P = 0.028), diastolic blood pressure (P = 0.005), RR interval (P = 0.020), PR interval (P = 0.040), and stress score (P = 0.027) in first time participants. Similarly, in the second group (repeaters) also significant difference was noticed. Conclusion: AMP has its positive effects on ECG, blood pressure, and stress level. Thus, it can be considered as one of the important nonpharmacological methods for prevention of stress, anxiety, and cardiovascular diseases.