An effective universal protocol for the extraction of fructooligosaccharide from the different agricultural byproducts
Ramachandran Chelliah,
Se Jin Park,
Sungyoon Oh,
Eunseok Lee,
Eric Banan-Mwine Daliri,
Fazle Elahi,
Chae Rin Park,
Ghazala Sultan,
Inamul Hasan Madar,
Deog Hwan Oh
Affiliations
Ramachandran Chelliah
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea; Department of Food Science and Biotechnology, Kangwon Institute of Inclusive Technology (KIIT), Kangwon National University, Gangwon-do 24341, Republic of Korea; Saveetha School of Engineering, (SIMATS), Chennai, Tamil Nadu 600124, India
Se Jin Park
School of Natural Resources and Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
Sungyoon Oh
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea
Eunseok Lee
School of Natural Resources and Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
Eric Banan-Mwine Daliri
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea; Department of Biological Models, Institute of Biochemistry, Life Science Center, Vilnius University, Saul..tekio al. 7, 10257 Vilnius. Lithuania
Fazle Elahi
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea
Chae Rin Park
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea
Ghazala Sultan
Department of Computer Science, Aligarh Muslim University, Aligarh 202002, India
Inamul Hasan Madar
Department of Pharmacology, Saveetha Dental College and Hospitals, Chennai, Tamil Nadu 600077, India
Deog Hwan Oh
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea; Corresponding author.
Alternative bio-refinery technologies are required to promote the commercial utilization of plant biomass components. The fructooligosaccharide (FOS) obtained after hydrolysis of the hemicellulose fractions was mainly applied in the pharmaceutical and food industries. Agricultural bi-product is a rich constituent in dietary fibres, which have prebiotic effects on the intestinal microbiota and the host. Herein we explored the impact of FOS on microbiota modulation and the gut homeostasis effect. High fructooligosaccharide recovery was obtained using alkaline extraction techniques. The enzymatic method produced fructooligosaccharides with minor contamination from fructan and glucan components, although it had a low yield. But combining the alkaline and enzymatic process provides a higher yield ratio and purity of fructooligosaccharides. The structure of the fructooligosaccharide was confirmed, according to FTIR, 13C NMR, 1H NMR and 2D-NMR data. Our results could be applied to the development of efficient extraction of valuable products from agricultural materials using enzyme-mediated methods, which were found to be a cost-effective way to boost bio-refining value. Fructooligosaccharides with varying yields, purity, and structure can be obtained.
