Study on potential of some solid natural substances in production of Beauveria bassiana (Ascomycota, Cordycipitaceae) conidia

Abstract

In this study, two fungus isolates, Beauveria bassiana EUT105 and B. bassiana EUT116, were evaluated for production of aerial conidia on wheat, wheat flour, wheat bran, rice flour, rice bran, rice paddy, millet and corn flour using two phasic liquid-solid system. Data analysis showed that there are significant differences among treatments (simple media) in both isolates. In B. bassiana EUT105, the maximum and minimum productions were achieved on wheat bran, 2.3 × 1010 conidia/g and on rice paddy, 1.5 × 109 conidia/g of medium, respectively. In B. bassiana EUT116, wheat bran with 6.1 × 109 conidia/g and millet with 8 × 108 conidia/g had the maximum and minimum productions, respectively. Effect of adding nutritional complementary compounds to the growth media was significant in increasing of conidial yield. Related results showed that adding permeate increased the production but molasses did not it in comparison with control in both isolates. Wheat bran plus permeate had the maximum production, 5 × 1010 and 4.6 × 1010 conidia/g in B. bassiana EUT105 and EUT116, respectively and minimum production was obtained with rice flour plus molasses, 1.5 × 108 and 6 × 108 conidia/g in B. bassiana EUT105 and EUT116, respectively.

Keywords

• beauveria bassiana
• natural medium
• conidial production
• complementary substance
• entomopathogenic fungus