Laccase-based catalytic microreactor for BPA biotransformation
Juan Eduardo Sosa-Hernández,
Elsa M. Gutierrez,
Jhosseph S. Ochoa Sierra,
Osvaldo Aquines,
Felipe Robledo-Padilla,
Elda M. Melchor-Martínez,
Hafiz M.N. Iqbal,
Roberto Parra-Salvídar
Affiliations
Juan Eduardo Sosa-Hernández
Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, 64849, Mexico; Tecnologico de Monterrey, Institute of Advanced Materials for Sustainable Manufacturing Monterrey, 64849, Mexico; Corresponding author. Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, 64849, Mexico.
Elsa M. Gutierrez
Departmento de Ingeniería Biomédica, Universidad de Monterrey, Monterrey, Nuevo León, Mexico
Jhosseph S. Ochoa Sierra
Escuela de Microbiología, Universidad de Antioquia, Medellín, Antioquia, Colombia
Osvaldo Aquines
Department of Physics and Mathematics, Universidad de Monterrey, San Pedro Garza García, Mexico
Felipe Robledo-Padilla
Department of Physics and Mathematics, Universidad de Monterrey, San Pedro Garza García, Mexico
Elda M. Melchor-Martínez
Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, 64849, Mexico; Tecnologico de Monterrey, Institute of Advanced Materials for Sustainable Manufacturing Monterrey, 64849, Mexico
Hafiz M.N. Iqbal
Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, 64849, Mexico; Tecnologico de Monterrey, Institute of Advanced Materials for Sustainable Manufacturing Monterrey, 64849, Mexico
Roberto Parra-Salvídar
Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, 64849, Mexico; Tecnologico de Monterrey, Institute of Advanced Materials for Sustainable Manufacturing Monterrey, 64849, Mexico; Corresponding author. Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, 64849, Mexico.
A laccase-based catalytic reactor was developed into a polydimethylsiloxane (PDMS) microfluidic device, allowing the degradation of different concentrations of the emergent pollutant, Bisphenol-A (BPA), at a rate similar to free enzyme. Among the immobilizing agents used, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was capable of immobilizing a more significant amount of the laccase enzyme in comparison to glutaraldehyde (GA), and the passive method (2989, 1537, and 1905 U/mL, respectively). The immobilized enzyme inside the microfluidic device could degrade 55 ppm of BPA at a reaction rate of 0.5309 U/mL*min with a contaminant initial concentration of 100 ppm at room temperature. In conclusion, the design of a microfluidic device and the immobilization of the laccase enzyme successfully allowed a high capacity of BPA degradation.
