The Histological Changes in Endocrine Part of the Pancreas of Diabetic Rats Treated with Rosa Damascena Extract

Abstract

Background: Diabetes is caused by a breakdown in insulin production or its malfunction in the body. So far, many studies have been done on its treatment. The aim of this study was to investigate the histological changes in endocrine part of the pancreas of diabetic rats treated with Rosa damascena extract. Methods: In this experimental study, 100 male rats were divided into 10 groups of control, untreated diabetic, gavage stress, insulin treatment, three healthy groups receiving different doses of extract, and three diabetic groups receiving different doses of extract (300, 600, and 900 mg/kg). Finally, pancreatic tissue was removed and immunohistochemically stained with hematoxylin-eosin to count pancreatic beta cells number, and to calculate islets of Langerhans. Statistical analysis was performed using ANOVA test, and P < 0.050 was considered as statistically significant. Findings: Rosa damascena extract at 900 mg/kg, significantly increased the area of the islets of Langerhans in diabetic rats compared to the diabetic control group (P = 0.021). This dose of extract also increased the percentage of beta cells in the islets of Langerhans compared to the diabetic control group (P = 0.017). Conclusion: According to the results, using rose extract can improve tissue changes in the pancreas. This extract can probably be considered as a suitable research potential in future studies.

Keywords

• diabetes mellitus
• rosa
• insulin
• blood glucose