Disinfection Efficacy of Laser Activation on Different Forms and Concentrations of Sodium Hypochlorite Root Canal Irrigant against <i>Enterococcus faecalis</i> in Primary Teeth
Chandrashekar Murugesh Yavagal,
Srinivas K. Subramani,
Viplavi Chavan Patil,
Puja C. Yavagal,
Ramachandra P. Talwar,
Mamata Iranna Hebbal,
Selma A. Saadaldin,
Elzahraa Eldwakhly,
Manal M. Abdelhafeez,
Mai Soliman
Affiliations
Chandrashekar Murugesh Yavagal
Department of Pediatric Dentistry, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences & Research Centre, Belgaum 590019, Karnataka, India
Srinivas K. Subramani
Department of Pediatric Dentistry, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences & Research Centre, Belgaum 590019, Karnataka, India
Viplavi Chavan Patil
Department of Pediatric Dentistry, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences & Research Centre, Belgaum 590019, Karnataka, India
Puja C. Yavagal
Department of Public Health Dentistry, Bapuji Dental College and Hospital, Davanagere 577004, Karnataka, India
Ramachandra P. Talwar
Department of Pediatric Dentistry, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences & Research Centre, Belgaum 590019, Karnataka, India
Mamata Iranna Hebbal
Department of Preventive Dental Sciences, College of Dentistry, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Selma A. Saadaldin
Prosthodontics Division, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 3K7, Canada
Elzahraa Eldwakhly
Department of Clinical Dental Sciences, College of Dentistry, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Manal M. Abdelhafeez
Department of Conservative Dental Sciences, College of Dentistry, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia
Mai Soliman
Department of Clinical Dental Sciences, College of Dentistry, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Photoactivated disinfection with sodium hypochlorite (NaOCl) has improved primary root canal treatment outcomes. This in vitro study aims to assess and compare the disinfecting efficacy of 2.5% sodium hypochlorite solution and 5.25% sodium hypochlorite gel, without laser activation and accompanied by laser activation, on Enterococcus faecalis-contaminated primary teeth root canals. After one month of incubating extracted teeth specimens with E. faecalis, 36 specimens were randomly divided into two groups: Group A (conventional method without laser-activated irrigation) and Group B (with laser-activated irrigation). Each group was further divided into three subgroups, with six samples in each subgroup. Subgroup 1 received irrigation with normal saline, Subgroup 2 with 2.5% sodium hypochlorite solution, and Subgroup 3 with 5.25% sodium hypochlorite gel. Diode laser activation at 810 nm was used in Group B. Bacterial colony counts were measured before and after the intervention. Student’s t-test and one-way analysis of variance (ANOVA) with Tukey’s post hoc test were used for statistical analysis. The significance level was set at p p = 0.02) improved the disinfection ability of the irrigant compared to the non-activation group. The disinfection ability of sodium hypochlorite gel was better than that of saline (p = 0.02); however, it was comparable to that of sodium hypochlorite solution (p = 0.67). Conclusion: Root canal irrigation with 5.25% sodium hypochlorite gel activated with an 810 nm diode laser resulted in complete eradication of Enterococcus faecalis, indicating its effectiveness as an endodontic disinfection treatment modality.
